Hericenone A
Hericenone A is an isoindolinone found in the fruiting bodies of Hericium erinaceus. Hericenone A exerts cytotoxic activity against cancer cells. Hericenone A can be used for the research of cancer, such as metastatic human hepatocellular carcinoma.
For research use only. We do not sell to patients.
- CAS No.: 126654-52-2
- Formula: C19H22O5
- Molecular Weight:330.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Hericenone A (compound 6) (5-20 μg/mL; 24 h) dose-dependently inhibits the growth of SMMC-7221 and MHCC-97H human hepatocellular carcinoma cells, with the most potent activity observed at 20 μg/mL for 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SMMC-7221, MHCC-97H
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Concentration:5, 10, 20 μg/mL
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Incubation Time:24 h
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Result:Inhibited the growth of SMMC-7221 cells in a dose-dependent manner.
Inhibited the growth of MHCC-97H cells in a dose-dependent manner.
Showed the most potent activity against both cell lines at 20 μg/mL among all tested compounds.
Chemical Information
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CAS No. 126654-52-2
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Molecular Weight 330.37
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Formula C19H22O5
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SMILES
OC1=C2C(C(OC2)=O)=CC(OC)=C1C/C=C(C)/CC(/C=C(C)\C)=O
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Structure Classification
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Initial Source
Hericium erinaceus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Hericenone A Related Classifications
HY-N15388 Related Classifications
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