GFOGER peptide 

GFOGER peptide is an artificially synthesized collagen-mimetic sequence. GFOGER peptide acts as a ligand for α2β1, α11β1 and α1β1 integrins, thereby supporting integrin-mediated cell adhesion to collagen. GFOGER peptide triggers signaling pathways mediated by the α2β1 integrin receptor and upregulates osteoblast differentiation. GFOGER peptide accelerates and enhances bone formation at sites of refractory femoral defects. GFOGER peptide can be passively adsorbed onto polymer scaffolds for cell-free/growth factor-free bone formation. GFOGER peptide is used in biomaterials such as hydrogels and 3D bioinks for tissue engineering research including bone formation^[1][2][3].

GFOGER peptide (20 μg/mL; overnight at 4°C coating, 1 h binding) is bound with high affinity by recombinant human integrin α₁₁ I domain in a Mg²⁺-dependent solid-phase binding assay^[2].
GFOGER peptide (500 μM; 8-10 days, 24 h, 72 h) inhibits 4 mM Pi-induced calcification in MOVAS-1 cells by 66%, does not reduce cell viability, normalizes Pi-induced upregulation of osteogenic marker genes (Runx2, MGP, OCN, TNAP) after 8 days, and normalizes SMPD3 gene expression after 8 days of treatment^[3].
GFOGER peptide (500 μM; 14 days) inhibits 4 mM Pi-induced calcification in primary human VSMCs by 58%^[3].
GFOGER peptide (500 μM; 10 days) modifies the protein content of 4 mM Pi-treated MOVAS-1 cell-derived EVs, normalizing the expression of most Pi-altered proteins involved in vascular calcification^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

After passive adsorption of GFOGER peptide (50 μg/mL; approximately 260 ng per 9 mm scaffold; implanted; single administration) onto PCL scaffolds, compared with uncoated scaffolds, the peptide significantly accelerates and increases bone volume at the site of critical-sized femoral defects in rats (enhanced defect bridging)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

677.71

C₂₉H₄₃N₉O₁₀

245120-10-9

Solid

White to off-white

Gly-Phe-{Hyp}-Gly-Glu-Arg

GF-{Hyp}-GER

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Wojtowicz AM, et al. Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair. Biomaterials. 2010;31(9):2574-2582.

  [2]. Zhang WM, et al. alpha 11beta 1 integrin recognizes the GFOGER sequence in interstitial collagens. J Biol Chem. 2003 Feb 28;278(9):7270-7.  [Content Brief]

  [3]. Mansour A, et al. GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification. Front Cell Dev Biol. 2020;8:589761. Published 2020 Nov 30.  [Content Brief]