Glycerol-3-phosphate oxidase, E. coli  (Synonyms: GPO)

Glycerol-3-phosphate oxidase, E. coli (GPO) is a key intermediate in glycerol metabolism. Glycerol-3-phosphate oxidase, E. coli is the skeleton of phospholipids in membrane lipids, and also a substrate in the respiratory chain. Glycerol-3-phosphate oxidase, E. coli produces electrons through oxidation. Glycerol-3-phosphate oxidase, E. coli is an enzyme with important functions in the field of biochemistry, and is widely used in medical testing and scientific research^[1][2][3].

Glycerol-3-phosphate oxidase, E. coli catalyzes the oxidation of glycerol 3-phosphate to dihydroxyacetone phosphate (DHAP), accompanied by the reduction of O₂ to H₂O₂. Hydrogen peroxide generated by this enzyme is critical for the cytotoxicity of Mycoplasma pneumoniae against host cells. It participates in glycerol metabolism and is indispensable for glycerol utilization^[2].
Glycerol-3-phosphate oxidase, E. coli can utilize glyceraldehyde 3-phosphate (GAP), a glycolytic intermediate, as a substrate, though the structure of its catalytic product remains uncharacterized. Accordingly, this enzyme may produce hydrogen peroxide in mycoplasma infection when glycerol is unavailable^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9046-28-0

Solid

Light yellow to yellow

[Glycerol-3-phosphate oxidase, E. coli]

1.1.3.21

≥25 U/mg protein

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH 7.0.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Koga Y, et al. Anaerobic glycerol-3-phosphate dehydrogenase complex from hyperthermophilic archaeon Thermococcus kodakarensis KOD1. J Biosci Bioeng. 2019;127(6):679-685.  [Content Brief]

  [2]. Hames C, et al. Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae. J Bacteriol. 2009 Feb;191(3):747-53.

  [3]. Maenpuen S, et al. Kinetic mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae. FEBS J. 2015 Aug;282(16):3043-59.