GPC3-targeting peptide 2, scrambled control
GPC3-targeting peptide 2, scrambled control serves as the scrambled negative control for GPC3-targeting peptide 2. GPC3-targeting peptide 2, scrambled control can be used as a control peptide to verify the specific binding of GPC3-targeting peptides to cancer cells and tumors overexpressing GPC3.
For research use only. We do not sell to patients.
- Formula: C62H96N16O20
- Molecular Weight:1385.52
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
GPC3-targeting peptide 2, scrambled control (FEAEHNLALQTL) (5 μM; 5 min) shows minimal, non-specific binding to GPC3-expressing human Hep3B hepatocellular carcinoma cells, with no significant change in binding upon GPC3 knockdown[1].
GPC3-targeting peptide 2, scrambled control (50-200 μM; 30 min) does not compete with GPC3-targeting ALL*-IRDye800 for binding to human Hep3B hepatocellular carcinoma cells at concentrations up to 200 μM[1].
GPC3-targeting peptide 2, scrambled control (5 μM; 5 min) shows minimal non-specific binding to GPC3-negative human SK-Hep-1 hepatocellular carcinoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human Hep3B hepatocellular carcinoma cells (GPC3-expressing) transfected with control siRNA (siCL) or GPC3-targeting siRNAs
-
Concentration:5 μM
-
Incubation Time:5 min (room temperature)
-
Result:Showed minimal fluorescence signal in Hep3B cells transfected with control siRNA.
Exhibited no significant reduction in fluorescence intensity in cells transfected with any of the 3 GPC3-targeting siRNAs compared to control siRNA-transfected cells.
Demonstrated fluorescence intensity 4.7-fold lower than that of the GPC3-targeting ALL*-IRDye800 peptide in control siRNA-transfected Hep3B cells.
-
Cell Line:human Hep3B hepatocellular carcinoma cells
-
Concentration:50-200 μM (unlabeled peptide); 5 μM (IRDye800-labeled ALL* peptide)
-
Incubation Time:30 min (room temperature, unlabeled peptide); 30 min (4 °C, IRDye800-labeled ALL* peptide)
-
Result:Caused no significant change in the fluorescence intensity of IRDye800-labeled ALL* peptide bound to Hep3B cells at concentrations ranging from 50 to 200 μM.
-
Cell Line:human SK-Hep-1 hepatocellular carcinoma cells (GPC3-negative)
-
Concentration:5 μM
-
Incubation Time:5 min (room temperature)
-
Result:Showed minimal fluorescence signal binding to SK-Hep-1 cells.
Exhibited no significant difference in intensity compared to the GPC3-targeting ALL*-IRDye800 peptide.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:nu/nu (female, 4-6 weeks old, 20-25 g, orthotopically implanted with human Hep3B HCC cells)[1]
-
Dosage:150 μM
-
Administration:i.v.
-
Result:Showed minimal tumor fluorescence intensity over 48 h, with a tumor-to-background (T/B) ratio that remained near baseline.
Demonstrated a mean T/B ratio significantly lower than that of the GPC3-targeting peptide ALL*-IRDye800 via laparoscopic imaging at 1.5 h post-injection.
Exhibited a mean T/B ratio of ~1.1 via whole-body fluorescence imaging at 1.5 h post-injection, significantly lower than the ~2.0 T/B ratio of ALL*-IRDye800.
Showed minimal tumor fluorescence intensity compared to ALL*-IRDye800 via biodistribution analysis, with no significant difference in signal between tumor and adjacent liver tissue.
Chemical Information
-
Molecular Weight 1385.52
-
Formula C62H96N16O20
-
Sequence
Phe-Glu-Ala-Glu-His-Asn-Leu-Ala-Leu-Gln-Thr-Leu
-
Sequence Shortening
FEAEHNLALQTL
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
GPC3-targeting peptide 2, scrambled control Related Classifications
HY-P11938 Related Classifications
Powered by Bioz