Cathayanon J
Cathayanon J is a flavanone natural product with 2R configuration, which is found in the stem bark of Morus cathayana. Cathayanon J exhibits weak cytotoxicity against A549, Bel 7402, BGC-823, HCT-8 and A2780 human cancer cells, with IC50 values of 4.67, 5.95, 6.70, 5.86 and 5.79 μg/mL, respectively. Cathayanon J can be used in cancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 1303438-53-0
- Formula: C30H36O5
- Molecular Weight:476.60
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
4.67 μg/mL
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Cytotoxicity against human lung carcinoma A549 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human lung carcinoma A549 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| Bel-7402 | IC50 |
5.95 μg/mL
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Cytotoxicity against human liver carcinoma Bel7402 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human liver carcinoma Bel7402 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| BGC-823 | IC50 |
6.70 μg/mL
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Cytotoxicity against human stomach carcinoma BGC-823 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human stomach carcinoma BGC-823 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| HCT-8 | IC50 |
5.86 μg/mL
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Cytotoxicity against human colon carcinoma HCT-8 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human colon carcinoma HCT-8 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| A2780 | IC50 |
5.79 μg/mL
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Cytotoxicity against human ovarian carcinoma A2780 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human ovarian carcinoma A2780 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
In Vitro
Cathayanon J (0.1-10.0 μg/mL; 4 days) exhibits cytotoxicity against human cancer cell lines A549, Bel 7402, BGC-823, HCT-8 and A2780, with IC50 values of 4.67, 5.95, 6.70, 5.86 and 5.79 μg/mL, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human lung carcinoma (A549), human liver carcinoma (Bel7402), human stomach carcinoma (BGC-823), human colon carcinoma (HCT-8), human ovarian carcinoma (A2780)
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Concentration:0.1, 1.0, 10.0 μg/mL
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Incubation Time:4 days
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Result:Exhibited weak cytotoxic activity against human lung carcinoma (A549) cell line with an IC50 value of 4.67 μg/mL.
Exhibited weak cytotoxic activity against human liver carcinoma (Bel7402) cell line with an IC50 value of 5.95 μg/mL.
Exhibited weak cytotoxic activity against human stomach carcinoma (BGC-823) cell line with an IC50 value of 6.70 μg/mL.
Exhibited weak cytotoxic activity against human colon carcinoma (HCT-8) cell line with an IC50 value of 5.86 μg/mL.
Exhibited weak cytotoxic activity against human ovarian carcinoma (A2780) cell line with an IC50 value of 5.79 μg/mL.
Chemical Information
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CAS No. 1303438-53-0
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Molecular Weight 476.60
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Formula C30H36O5
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SMILES
O=C1C2=C(O)C=C(O)C=C2O[C@@H](C3=CC(C/C=C(C)/CC/C=C(C)\C)=C(C(C/C=C(C)\C)=C3)O)C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cathayanon J Related Classifications
HY-N21864 Related Classifications
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