IMP-2552

IMP-2552 (Compound 10) (2 h) potently inhibits the RAB27A-effector protein-protein interaction in a cell-free fluorescence polarization assay with an IC₅₀ of 3.61 μM and a k_inact/K_i of 32.1/M·s^[1].
IMP-2552 (10 μM; 1 h) covalently and selectively modifies the Cys123 residue of recombinant RAB27A and RAB27B with equal potency against both active (GTP-bound) and inactive (GDP-bound) RAB27A, achieving 82.3% labeling of nRAB27A-Cys123 with minimal modification of Cys188^[1].
IMP-2552 (10 μM; 2 h pre-incubation) stereoselectively engages endogenous RAB27A and RAB27B in MDA-MB-231 cells, competing for probe binding without affecting off-target proteins or inducing cytotoxicity^[1].
IMP-2552 (10-20 μM; 14 h) disrupts the endogenous RAB27A-SLP2-A protein-protein interaction in MDA-MB-231 cells^[1].
IMP-2552 (10 μM; 24 h) induces perinuclear melanosome clustering in melan-a mouse melanocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.