IMP-2958
IMP-2958 is an inactive enantiomeric negative control probe with significantly reduced binding to RAB27A/B-Cys123.IMP-2958 serves as a negative control probe for off-target binding in phenotypic assays related to RAB27A/B studies.IMP-2958 functions as a control for stereoselective targeted covalent inhibitors of the RAB27-effector protein-protein interaction.
For research use only. We do not sell to patients.
- Formula: C25H26N6O5S
- Molecular Weight:522.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IMP-2958 does not inhibit the WF pocket interaction of nRAB27ACys123, with an IC50 > 100 μM in the FP competition assay[1].
IMP-2958 (10 μM; 1 h) shows minimal covalent engagement of nRAB27A-Cys123, with only 7.7% labeling after 1-hour incubation at 10 μM[1].
IMP-2958 (10 μM; 2 h) does not engage endogenous RAB27A or RAB27B in MDA-MB-231 cells at 10 μM, as it fails to compete with probe IMP-2566 for target binding[1].
IMP-2958 (10-20 μM; 14 h) does not disrupt the RAB27A-SLP2-A protein-protein interaction in MDA-MB-231 cells at concentrations up to 20 μM after 14-hour incubation[1].
IMP-2958 (10 μM; 24 h) does not phenocopy RAB27A knockout in melan-a mouse melanocytes at 10 μM after 24-hour incubation, as it does not induce perinuclear melanosome accumulation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 522.58
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Formula C25H26N6O5S
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SMILES
O=C([C@@H]1CCCN1S(C2=CC=C(NC(C=C)=O)C=C2)(=O)=O)NC3=CC=C(C(NC4=NN(C)C=C4)=O)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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