Isobutylamido thiazolyl resorcinol
Based on 1 Customer Validation
Isobutylamido thiazolyl resorcinol is a selective hTyr inhibitor with an IC50 of 1.1 μM. Isobutylamido thiazolyl resorcinol shows an IC50 of 108 μM against mushroom Tyrosinase. Isobutylamido thiazolyl resorcinol effectively prevents pigmentation caused by UVB irradiation. Isobutylamido thiazolyl resorcinol significantly improves the visibility of acne-induced hyperpigmentation.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 1428450-95-6
- Formula: C13H14N2O3S
- Molecular Weight:278.33
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 1.1 μM (human Tyrosinase); 108 μM (mushroom Tyrosinase)[3]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MNT-1 | CC50 |
>100 μM
Compound: Thiamidol
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Cytotoxicity against human MNT-1 cells incubated for 4 days by resazurin assay
Cytotoxicity against human MNT-1 cells incubated for 4 days by resazurin assay
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[PMID: 38262119] |
| MNT-1 | CC50 |
36 μM
Compound: Thiamidol
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Cytotoxicity against human MNT-1 cells incubated for 14 days by resazurin assay
Cytotoxicity against human MNT-1 cells incubated for 14 days by resazurin assay
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[PMID: 38262119] |
| MNT-1 | IC50 |
0.28 μM
Compound: Thiamidol
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Inhibition of melanogenesis in human MNT-1 cells incubated for 14 days
Inhibition of melanogenesis in human MNT-1 cells incubated for 14 days
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[PMID: 38262119] |
| MNT-1 | IC50 |
10 μM
Compound: Thiamidol
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Inhibition of melanogenesis in human MNT-1 cells incubated for 4 days
Inhibition of melanogenesis in human MNT-1 cells incubated for 4 days
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[PMID: 38262119] |
Isobutylamido thiazolyl resorcinol strongly but reversibly inhibits melanin production in melanocyte cultures (IC50 = 0.9 μM)[1].
Isobutylamido thiazolyl resorcinol significantly improves the visibility of acne-induced hyperpigmentation[2].
Isobutylamido thiazolyl resorcinol strongly inhibits hTyr (IC50 = 1.1 μM), while weakly suppresses mushroom Tyr activity (IC50 = 108 μM)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1428450-95-6
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Appearance Solid
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Molecular Weight 278.33
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Formula C13H14N2O3S
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Color White to off-white
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SMILES
O=C(C(C)C)NC1=NC(C2=C(O)C=C(O)C=C2)=CS1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (359.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (17.96 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (17.96 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Vachiramon V, et al. Isobutylamido thiazolyl resorcinol for prevention of UVB-induced hyperpigmentation. J Cosmet Dermatol. 2021 Mar;20(3):987-992. [Content Brief]
[2]. Roggenkamp D, et al. Effective reduction of post-inflammatory hyperpigmentation with the tyrosinase inhibitor isobutylamido-thiazolyl-resorcinol (Thiamidol). Int J Cosmet Sci. 2021 Jun;43(3):292-301. [Content Brief]
[3]. Mahalapbutr P, et al. Structural dynamics and susceptibility of isobutylamido thiazolyl resorcinol (ThiamidolTM) against human and mushroom tyrosinases. J Biomol Struct Dyn. 2023;41(21):11810-11817. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5929 mL | 17.9643 mL | 35.9286 mL | 89.8214 mL |
| 5 mM | 0.7186 mL | 3.5929 mL | 7.1857 mL | 17.9643 mL | |
| 10 mM | 0.3593 mL | 1.7964 mL | 3.5929 mL | 8.9821 mL | |
| 15 mM | 0.2395 mL | 1.1976 mL | 2.3952 mL | 5.9881 mL | |
| 20 mM | 0.1796 mL | 0.8982 mL | 1.7964 mL | 4.4911 mL | |
| 25 mM | 0.1437 mL | 0.7186 mL | 1.4371 mL | 3.5929 mL | |
| 30 mM | 0.1198 mL | 0.5988 mL | 1.1976 mL | 2.9940 mL | |
| 40 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL | 2.2455 mL | |
| 50 mM | 0.0719 mL | 0.3593 mL | 0.7186 mL | 1.7964 mL | |
| 60 mM | 0.0599 mL | 0.2994 mL | 0.5988 mL | 1.4970 mL | |
| 80 mM | 0.0449 mL | 0.2246 mL | 0.4491 mL | 1.1228 mL | |
| 100 mM | 0.0359 mL | 0.1796 mL | 0.3593 mL | 0.8982 mL |
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