JBSNF-000028 free base

Name: JBSNF-000028 free base
Brand: MedChemExpress
SKU: HY-151500
Rating: 4.5 (0 reviews)

製品番号: HY-151500 純度: 99.20%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

JBSNF-00002 free base is an orally active small molecule inhibitor of the tricyclic nicotinamide N-methyltransferase (NNMT) with IC₅₀ values for human, mouse and monkey sources of NNMT of 33, 210 and 190 nM respectively. JBSNF-00002 free base can reduce endogenous MNA levels in U2OS osteosarcoma cells, with its EC₅₀ being 2.5 μM. JBSNF-00002 free base exhibits significant anti-obesity and anti-diabetic activities in the diet-induced obesity (DIO) model. JBSNF-00002 free base can be used for the study of metabolic diseases such as obesity, type 2 diabetes and non-alcoholic fatty liver disease.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

JBSNF-000028 free base 構造式

CAS 番号 : 3026599-90-3

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 396	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 396	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$360	在庫あり	Estimated Time of Arrival: December 31
10 mg	$570	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of JBSNF-000028 free base:

JBSNF-000028 TFA 在庫あり
JBSNF-000028 hydrochloride 在庫あり
JBSNF-000028 お問い合わせ

Amine N-methyltransferase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

PNMT NNMT

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

IC₅₀: 0.033 µM (hNNMT), 0.19 µM (mkNNMT), 0.21 µM (mNNMT)^[1]

体外実験

JBSNF-000028 (24 h) inhibits NNMT activity with an EC₅₀ of 2.5 μM in U2OS cells^[1].
JBSNF-000028 (10-100 μM; 72 h) has no cytotoxicity against HepG2 cells^[1].
JBSNF-000028 binds below a hairpin structural motif at the nicotinamide pocket and stacks between Tyr-204 (from Hairpin) and Leu-164 (from central domain)^[1].
JBSNF-000028 is inactive against a broad panel of targets related to metabolism and safety^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

JBSNF-000028 (50 mg/kg; p.o.; twice daily for 27 days) improves glucose and lipid handling in mice with diet-induced obesity (DIO)^[1].
JBSNF-000028 (50 mg/kg; p.o.; twice daily for 4 weeks) improves glucose tolerance in NNMT knockout mice with diet-induced obesity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL6/N mice, diet induced obesity (DIO) model^[1]
Dosage:	50 mg/kg
Administration:	Oral administration, b.i.d for 27 days
Result:	Significantly reduced the body weight and fed blood glucose. Reduced 1-methyl-nicotinamide (MNA) levels in visceral WAT and liver. Led to a statistically significant reduction in plasma triglyceride, plasma LDL cholesterol, liver triglyceride and liver total cholesterol.

Animal Model:

Male C57BL6/N mice^[1]

Dosage:

1 mg/kg and 10 mg/kg

Administration:

Intravenous and oral administration (Pharmacokinetic Analysis)

Result:

Pharmacokinetic parameters of JBSNF-0000028 in mice following intravenous (1 mg/kg) and oral administration (10 mg/kg)^[1].

PK parameters	Intravenous	Oral
Dose (mg/kg)	1	10
AUC_0–t (ng h/mL)	446	1369
C₀/C_max (ng/mL)	432	452
T_max (h)	-	1.00
T_1/2 (h)	1.77	2.36
T_last (h)	8.00	10.0
Cl (mL/min/kg)	36.6	-
Vd (L/kg)	8.69	-
F (%)	-	30

分子量

187.24

分子式

C₁₁H₁₃N₃

CAS 番号

3026599-90-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

CN1CC2=C3C(CCN3C1=N)=CC=C2

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 60 mg/mL (320.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3407 mL	26.7037 mL	53.4074 mL
5 mM	1.0681 mL	5.3407 mL	10.6815 mL
10 mM	0.5341 mL	2.6704 mL	5.3407 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (16.02 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.20%

Select Batch:

データシート (279 KB) SDS (251 KB)

COA (251 KB) HNMR (243 KB) RP-HPLC (277 KB) LCMS (116 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Ruf S, et al. Novel tricyclic small molecule inhibitors of Nicotinamide N-methyltransferase for the treatment of metabolic disorders. Sci Rep. 2022 Sep 14;12(1):15440. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3407 mL	26.7037 mL	53.4074 mL	133.5185 mL
	5 mM	1.0681 mL	5.3407 mL	10.6815 mL	26.7037 mL
	10 mM	0.5341 mL	2.6704 mL	5.3407 mL	13.3518 mL
	15 mM	0.3560 mL	1.7802 mL	3.5605 mL	8.9012 mL
	20 mM	0.2670 mL	1.3352 mL	2.6704 mL	6.6759 mL
	25 mM	0.2136 mL	1.0681 mL	2.1363 mL	5.3407 mL
	30 mM	0.1780 mL	0.8901 mL	1.7802 mL	4.4506 mL
	40 mM	0.1335 mL	0.6676 mL	1.3352 mL	3.3380 mL
	50 mM	0.1068 mL	0.5341 mL	1.0681 mL	2.6704 mL
	60 mM	0.0890 mL	0.4451 mL	0.8901 mL	2.2253 mL
	80 mM	0.0668 mL	0.3338 mL	0.6676 mL	1.6690 mL
	100 mM	0.0534 mL	0.2670 mL	0.5341 mL	1.3352 mL