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製品番号: HY-135771

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Avarone

Name: Avarone
Brand: MedChemExpress
SKU: HY-135771
Availability: PreOrder

(別名: (+)-Avarone)

Avarone ((+)-Avarone) is a sesquiterpenoidal quinone that can be isolated from Dysidea avara. Avarone shows cytotoxicity. Avarone shows antileukemic activity.

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CAS 番号: 55303-99-6
分子式: C₂₁H₂₈O₂
分子量:312.45

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Avarone 構造式

CAS 番号: 55303-99-6

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生物活性
- 製品説明
- Cellular Effect
- 体外実験
- 体内実験
化学情報
プロトコル (3)
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

Avarone ((+)-Avarone) is a sesquiterpenoidal quinone that can be isolated from Dysidea avara. Avarone shows cytotoxicity. Avarone shows antileukemic activity^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	30.03 μM Compound: 1	Cytotoxicity against human A549 cells assessed as cell survival after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 26476666]
A549	IC₅₀	6 μM Compound: 2 (Avarone)	Cytotoxicity was measured against neoplastic cultured A-549 cells of human lung carcinoma. Cytotoxicity was measured against neoplastic cultured A-549 cells of human lung carcinoma.	10.1016/0960-894X(96)00326-5
A549	IC₅₀	6.4 μM Compound: Avarone	Cytotoxic activity against cultured cells of A-549 human lung carcinoma. Cytotoxic activity against cultured cells of A-549 human lung carcinoma.	[PMID: 11312925]
HaCaT	IC₅₀	37 μM Compound: 2	Cytotoxicity against human HaCaT cells after 72 hrs by MTT assay Cytotoxicity against human HaCaT cells after 72 hrs by MTT assay	[PMID: 27128177]
HeLa	IC₅₀	18.12 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as cell survival after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 26476666]
HeLa	IC₅₀	26.8 μM Compound: 2	Cytotoxicity against human HeLa cells assessed as decrease in total cellular protein by kenacid blue R binding method Cytotoxicity against human HeLa cells assessed as decrease in total cellular protein by kenacid blue R binding method	[PMID: 19995673]
HT-29	IC₅₀	6 μM Compound: 2 (Avarone)	Cytotoxicity was measured against neoplastic cultured HT-29 cells of human colon carcinoma. Cytotoxicity was measured against neoplastic cultured HT-29 cells of human colon carcinoma.	10.1016/0960-894X(96)00326-5
HT-29	IC₅₀	6.4 μM Compound: Avarone	Cytotoxic activity against cultured cells of HT-29 human colon carcinoma. Cytotoxic activity against cultured cells of HT-29 human colon carcinoma.	[PMID: 11312925]
K562	IC₅₀	17.08 μM Compound: 1	Cytotoxicity against human K562 cells assessed as cell survival after 72 hrs by MTT assay Cytotoxicity against human K562 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 26476666]
K562	IC₅₀	4.9 μM Compound: 2	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 19995673]
MDA-MB-453	IC₅₀	16.71 μM Compound: 1	Cytotoxicity against human MDA-MB-453 cells assessed as cell survival after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-453 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 26476666]
MRC5	IC₅₀	>200 μM Compound: 1	Cytotoxicity against human MRC5 cells assessed as cell survival after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as cell survival after 72 hrs by MTT assay	[PMID: 26476666]
NCI-H460	IC₅₀	24 μM Compound: 2	Cytotoxicity against multidrug resistant human NCI-H460 cells after 72 hrs by MTT assay Cytotoxicity against multidrug resistant human NCI-H460 cells after 72 hrs by MTT assay	[PMID: 27128177]
NCI-H460	IC₅₀	84 μM Compound: 2	Cytotoxicity against human NCI-H460 cells after 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells after 72 hrs by MTT assay	[PMID: 27128177]
P388	IC₅₀	3.1 μM Compound: 2 (Avarone)	Cytotoxicity was measured against neoplastic cultured P-388 cells of murine leukemia. Cytotoxicity was measured against neoplastic cultured P-388 cells of murine leukemia.	10.1016/0960-894X(96)00326-5
P388	IC₅₀	3.2 μM Compound: Avarone	Cytotoxic Activity against cultured cells of P-388 murine leukemia. Cytotoxic Activity against cultured cells of P-388 murine leukemia.	[PMID: 11312925]
PBMC	IC₅₀	4.5 μM Compound: 2	Cytotoxicity against human phytohemagglutininin-stimulated PBMC cells assessed as growth inhibition by MTT assay Cytotoxicity against human phytohemagglutininin-stimulated PBMC cells assessed as growth inhibition by MTT assay	[PMID: 19995673]
PBMC	IC₅₀	4.8 μM Compound: 2	Cytotoxicity against human PBMC cells by MTT assay Cytotoxicity against human PBMC cells by MTT assay	[PMID: 19995673]
SK-MEL-28	IC₅₀	6 μM Compound: 2 (Avarone)	Cytotoxicity was measured against neoplastic cultured MEL-28 cells of malign human melanoma. Cytotoxicity was measured against neoplastic cultured MEL-28 cells of malign human melanoma.	10.1016/0960-894X(96)00326-5
SK-MEL-28	IC₅₀	6.4 μM Compound: Avarone	Cytotoxic activity against cultured cells of MEL-28 malign human melanoma. Cytotoxic activity against cultured cells of MEL-28 malign human melanoma.	[PMID: 11312925]

体外実験

Avarone (24, 48, 72 h) inhibits U937 cell growth with IC₅₀s of 95.8, 52.9, 33.5 µM at 24, 48, 72 h, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Cytotoxicity Assay^[2]

View Protocol

Cell Line:

L5178Y, Melanoma, Hela, fibroblasts, human gingival cells
Concentration:
Incubation Time:
Result:

Showed cytotoxicity for L5178Y, Melanoma, Hela, fibroblasts, human gingival cells with ED₅₀s of 0.62, 26.4, 8.7, 11.3, 76.4 µM, respectively.

体内実験

Avarone (10 mg/kg; i.p.; once daily for 5 days) shows increases life span of leukemic mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

8-9 months, 32-35 g Male outbred NMIR mice (L5178Y cells)^[2]
Dosage:

10 mg/kg
Administration:

I.p.; once daily for 5 days
Result:

Increased life span over controls by 146% when treatment was begun 1 day after tumor implantation and by 87% when treatment was delayed until day 8.

化学情報

CAS 番号 55303-99-6
分子量 312.45
分子式 C₂₁H₂₈O₂
SMILES

O=C1C(C[C@]2(C)[C@@H](C)CC[C@]3(C)C(C)=CCC[C@@]23[H])=CC(C=C1)=O
別名

(+)-Avarone
Structure Classification
- Terpenoids
- Sesquiterpenes
- Quinones
- Benzene Quinones
Initial Source
- Marine natural products
Dysidea avara
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

プロトコル

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (276 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Sakurai J, et al. Highly efficient total synthesis of the marine natural products (+)-avarone, (+)-avarol, (-)-neoavarone, (-)-neoavarol and (+)-aureol. Chemistry. 2008;14(3):829-37. [Content Brief]

[2]. Müller WE, et al. Potent antileukemic activity of the novel cytostatic agent avarone and its analogues in vitro and in vivo. Cancer Res. 1985 Oct;45(10):4822-6. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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