Cathayanon J
Cathayanon J is a flavanone natural product with 2R configuration, which is found in the stem bark of Morus cathayana. Cathayanon J exhibits weak cytotoxicity against A549, Bel 7402, BGC-823, HCT-8 and A2780 human cancer cells, with IC50 values of 4.67, 5.95, 6.70, 5.86 and 5.79 μg/mL, respectively. Cathayanon J can be used in cancer-related research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 1303438-53-0
- 分子式: C30H36O5
- 分子量:476.60
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
4.67 μg/mL
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Cytotoxicity against human lung carcinoma A549 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human lung carcinoma A549 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| Bel-7402 | IC50 |
5.95 μg/mL
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Cytotoxicity against human liver carcinoma Bel7402 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human liver carcinoma Bel7402 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| BGC-823 | IC50 |
6.70 μg/mL
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Cytotoxicity against human stomach carcinoma BGC-823 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human stomach carcinoma BGC-823 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| HCT-8 | IC50 |
5.86 μg/mL
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Cytotoxicity against human colon carcinoma HCT-8 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human colon carcinoma HCT-8 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
| A2780 | IC50 |
5.79 μg/mL
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Cytotoxicity against human ovarian carcinoma A2780 cells assessed by MTT assay following 4-day incubation.
Cytotoxicity against human ovarian carcinoma A2780 cells assessed by MTT assay following 4-day incubation.
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10286020.2010.489817 |
体外実験
Cathayanon J (0.1-10.0 μg/mL; 4 days) exhibits cytotoxicity against human cancer cell lines A549, Bel 7402, BGC-823, HCT-8 and A2780, with IC50 values of 4.67, 5.95, 6.70, 5.86 and 5.79 μg/mL, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human lung carcinoma (A549), human liver carcinoma (Bel7402), human stomach carcinoma (BGC-823), human colon carcinoma (HCT-8), human ovarian carcinoma (A2780)
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Concentration:0.1, 1.0, 10.0 μg/mL
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Incubation Time:4 days
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Result:Exhibited weak cytotoxic activity against human lung carcinoma (A549) cell line with an IC50 value of 4.67 μg/mL.
Exhibited weak cytotoxic activity against human liver carcinoma (Bel7402) cell line with an IC50 value of 5.95 μg/mL.
Exhibited weak cytotoxic activity against human stomach carcinoma (BGC-823) cell line with an IC50 value of 6.70 μg/mL.
Exhibited weak cytotoxic activity against human colon carcinoma (HCT-8) cell line with an IC50 value of 5.86 μg/mL.
Exhibited weak cytotoxic activity against human ovarian carcinoma (A2780) cell line with an IC50 value of 5.79 μg/mL.
化学情報
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CAS 番号 1303438-53-0
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分子量 476.60
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分子式 C30H36O5
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SMILES
O=C1C2=C(O)C=C(O)C=C2O[C@@H](C3=CC(C/C=C(C)/CC/C=C(C)\C)=C(C(C/C=C(C)\C)=C3)O)C1
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Cathayanon J 関連分類
HY-N21864 関連分類
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