DMPE-PEG2000-N3
Based on 1 Customer Validation
DMPE-PEG2000-N3 (DMPE-PEG2000-Azide) is a conjugate composed of DMPE, PEG chains, and terminal azido groups (-N3). DMPE-PEG2000-Azide is a click chemistry reagent used for the targeted modification of liposomes/nanoparticles.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 94.87%
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
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Help & FAQs
Biological Activity
제품 설명
In Vitro
DMPE-PEG-Azide is used as a core component in the preparation of liposomes or lipid nanoparticles (LNPs) to encapsulate drugs or genes. Containing the Azide group, DMPE-PEG-Azide can undergo a copper-catalyzed azido-alkynylene cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo a ring-strain-driven alkynylene-azido cycloaddition reaction (SPAAC) with molecules containing DBCO or BCN groups.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Appearance Solid
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Color White to off-white
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SMILES
CCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCC)=O)COP(OCCNC(OCCOCCN=[N+]=[N-])=O)(O)=O)=O.[n]
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Synonyms
DMPE-PEG2000-Azide
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 25 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Protocol
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DMPE-PEG2000-N3 Related Classifications
HY-182836A Related Classifications
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