L&M-D-MR 

L&M-D-MR is a highly specific fluorescent "AND" logic probe with response moieties for leucine aminopeptidase (LAP) and monoamine oxidase (MAO). The coexistence of both LAP and MAO is required for L&M-D-MR to trigger intramolecular cyclization, release fluorophores and activate fluorescence. In the presence of only a single enzyme, L&M-D-MR generates only an extremely weak signal. L&M-D-MR enables bioimaging in living cells and mouse models, and can effectively distinguish different subtypes of liver diseases via blood samples or test strips. L&M-D-MR is widely used in studies related to liver cirrhosis, hepatitis B and drug-induced liver injury^[1][2][3][4].

L&M-D-MR (10 μM; 10 h) produces strong, selective fluorescence in MAO- and LAP-overexpressing HepG2 liver cancer cells, with a 13.0-fold higher fluorescence intensity ratio between HepG2 and QSG-7701 normal liver cells, and its response is ablated by pretreatment with MAO or LAP inhibitors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1015.12

C₅₄H₆₂N₈O₁₂

NCCCOC(N(C)C1=CC=C(COC(NCCN(CCNC(OCC2=CC=C(NC([C@@H](N)CC(C)C)=O)C=C2)=O)CC(NC3=CC=C(C4(OC(C5=C4C=CC=C5)=O)C(C=CC(OC)=C6)=C6O7)C7=C3)=O)=O)C=C1)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chen M, et al. A Molecular Logic Gate for Developing "AND" Logic Probes and the Application in Hepatopathy Differentiation. ACS Cent Sci. 2022;8(6):837-844.

  [2]. An X, et al. Dual-Locked Small Molecule Fluorescent Probe for Precise Disease Imaging. Chem Asian J. 2025;20(23):e00911.

  [3]. Li ZJ, et al. Recent Progress of Activity-Based Fluorescent Probes for Imaging Leucine Aminopeptidase. Biosensors (Basel). 2023;13(7):752. Published 2023 Jul 21.

  [4]. Li Y, et al. Recent Advances in Enzyme-Activated Dual-Locked Probes for Biological Applications. Chem Asian J. 2025;20(8):e202401647.