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MC3817 free base

MC3817 free base is a selective DNMT1 inhibitor. MC3817 free base inhibits DNMT1 and DNMT3A/3L with IC₅₀s of 0.044 μM and > 10μM, respectively. MC3817 free base inhibits P53-dependent cancer cell proliferation, induces apoptosis and DNA damage. MC3817 free base elevates cleaved Caspase 3, P53, and γH2AX. MC3817 free base can be used in non-small cell lung cancer, colon cancer, cervical cancer, triple-negative breast cancer and histiocytic lymphoma research.

For research use only. We do not sell to patients.

Formula: C₃₃H₃₇N₉O
Molecular Weight:575.71

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

MC3817 free base Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

DNMT1

0.044 μM (IC₅₀)

All DNA Methyltransferase Isoforms :

All Caspase Isoforms :

All DNA/RNA Synthesis Isoforms :

In Vitro

MC3817 (compound 14) (0.05-100 μM, 48 h) free base exhibits antiproliferative activity against A549 and H460 (non-small cell and lung cancer), HCT-116 (colon cancer), HeLa (cervical cancer), MDA-MB-231 (triple-negative breast cancer), and U937 (histiocytic lymphoma) cells^[1].
MC3817 (2 μM, 24-48 h) free base induces S/G2M phase arrest and triggers apoptotic cell death in HCT-116 cells^[1].
MC3817 (0.5-5 μM, 16-48 h) free base triggers DNA damage and activates the p53/γH2AX pathway, leading to cell-cycle arrest and apoptosis in HCT 116 cells^[1].
MC3817 (2.5 μM, 48 h) free base induces a much higher level of apoptosis in HCT-116 P53 WT cells compared with HCT-116 P53^-/- cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

A549 , H460, HCT-116, HeLa , MDA-MB 231, and U937
Concentration:

0.05-100 μM
Incubation Time:

48 h
Result:

Had IC₅₀ values of 5.5 μM, 6.8 μM, 0.4 μM, 4.2 μM, 1.9 μM and 5.5 μM for A549 cells, H460 cells, HCT-116 cells, HeLa cells, MDA-MB 231 cells, and U937 cells.

Cell Cycle Analysis^[1]

Cell Line:

HCT-116 cells
Concentration:

2 μM
Incubation Time:

48 h
Result:

Caused a block in the S/G2M phase associated with apoptotic cell death, affecting over 50% of the cells after 48 h.

Western Blot Analysis^[1]

Cell Line:

HCT-116 colon cancer cells
Concentration:

0.5, 1, 2.5, 5 μM
Incubation Time:

48 h
Result:

Induced the accumulation of γH2AX and elevated p53 levels, indicating p53 stabilization and activation in response to DNA damage.

Apoptosis Analysis^[1]

Cell Line:

HCT-116 P53^-/- cells and HCT-116 P53 WT cells.
Concentration:

2.5 μM
Incubation Time:

48 h
Result:

Induced apoptotic cell death, as evidenced by significant PI/Annexin V fluorescence and extensive membrane blebbing in both cell lines.

Chemical Information

Molecular Weight 575.71
Formula C₃₃H₃₇N₉O
SMILES

O=C(NC1=CC(CN(C)C)=CC(NC2=NC(N)=NC(C)=C2)=C1)C3=CC(CN(C)C)=CC(NC4=C5C=CC=CC5=NC=C4)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zwergel C, et al. Quinoline-Based DNA Methyltransferase Inhibitors Featuring Basic Side Chains: Design, Synthesis, and Insight in Biochemical and Anticancer Cell Properties. J Med Chem. 2025;68(22):23886-23909. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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MC3817 free base

All DNA Methyltransferase Isoforms

All Caspase Isoforms

All DNA/RNA Synthesis Isoforms