2. Epigenetics Apoptosis Cell Cycle/DNA Damage
  3. DNA Methyltransferase Caspase MDM-2/p53 DNA/RNA Synthesis Apoptosis
  4. MC3817 free base

MC3817 free base is a selective DNMT1 inhibitor. MC3817 free base inhibits DNMT1 and DNMT3A/3L with IC50s of 0.044 μM and > 10μM, respectively. MC3817 free base inhibits P53-dependent cancer cell proliferation, induces apoptosis and DNA damage. MC3817 free base elevates cleaved Caspase 3, P53, and γH2AX. MC3817 free base can be used in non-small cell lung cancer, colon cancer, cervical cancer, triple-negative breast cancer and histiocytic lymphoma research.

For research use only. We do not sell to patients.

MC3817 free base

MC3817 free base Chemical Structure

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MC3817 free base Related Antibodies

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DNA Methyltransferase DNMT1 DNMT3 MGMT

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

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Description

MC3817 free base is a selective DNMT1 inhibitor. MC3817 free base inhibits DNMT1 and DNMT3A/3L with IC50s of 0.044 μM and > 10μM, respectively. MC3817 free base inhibits P53-dependent cancer cell proliferation, induces apoptosis and DNA damage. MC3817 free base elevates cleaved Caspase 3, P53, and γH2AX. MC3817 free base can be used in non-small cell lung cancer, colon cancer, cervical cancer, triple-negative breast cancer and histiocytic lymphoma research[1].

IC50 & Target[1]

DNMT1

0.044 μM (IC50)

In Vitro

MC3817 (compound 14) (0.05-100 μM, 48 h) free base exhibits antiproliferative activity against A549 and H460 (non-small cell and lung cancer), HCT-116 (colon cancer), HeLa (cervical cancer), MDA-MB-231 (triple-negative breast cancer), and U937 (histiocytic lymphoma) cells[1].
MC3817 (2 μM, 24-48 h) free base induces S/G2M phase arrest and triggers apoptotic cell death in HCT-116 cells[1].
MC3817 (0.5-5 μM, 16-48 h) free base triggers DNA damage and activates the p53/γH2AX pathway, leading to cell-cycle arrest and apoptosis in HCT 116 cells[1].
MC3817 (2.5 μM, 48 h) free base induces a much higher level of apoptosis in HCT-116 P53 WT cells compared with HCT-116 P53-/- cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MC3817 free base Related Antibodies

Cell Viability Assay[1]

Cell Line: A549 , H460, HCT-116, HeLa , MDA-MB 231, and U937
Concentration: 0.05-100 μM
Incubation Time: 48 h
Result: Had IC50 values of 5.5 μM, 6.8 μM, 0.4 μM, 4.2 μM, 1.9 μM and 5.5 μM for A549 cells, H460 cells, HCT-116 cells, HeLa cells, MDA-MB 231 cells, and U937 cells.

Cell Cycle Analysis[1]

Cell Line: HCT-116 cells
Concentration: 2 μM
Incubation Time: 48 h
Result: Caused a block in the S/G2M phase associated with apoptotic cell death, affecting over 50% of the cells after 48 h.

Western Blot Analysis[1]

Cell Line: HCT-116 colon cancer cells
Concentration: 0.5, 1, 2.5, 5 μM
Incubation Time: 48 h
Result: Induced the accumulation of γH2AX and elevated p53 levels, indicating p53 stabilization and activation in response to DNA damage.

Apoptosis Analysis[1]

Cell Line: HCT-116 P53-/- cells and HCT-116 P53 WT cells.
Concentration: 2.5 μM
Incubation Time: 48 h
Result: Induced apoptotic cell death, as evidenced by significant PI/Annexin V fluorescence and extensive membrane blebbing in both cell lines.
Molecular Weight

575.71

Formula

C33H37N9O
SMILES

O=C(NC1=CC(CN(C)C)=CC(NC2=NC(N)=NC(C)=C2)=C1)C3=CC(CN(C)C)=CC(NC4=C5C=CC=CC5=NC=C4)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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MC3817 free base Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MC3817MC 3817MC-3817DNA MethyltransferaseCaspaseMDM-2/p53DNA/RNA SynthesisApoptosisDNMTsDNA MTasesDNMTDNMT3A/3Lcleaved Caspase 3P53γH2AXnon-small cell lung cancercolon cancercervical cancertriple-negative breast cancerhistiocytic lymphoma researchInhibitorinhibitorinhibit

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