Methyl Green Staining Solution (Chloroform-Extracted, 1%)

Methyl Green Staining Solution (Chloroform-Extracted, 1%) is a basic dye stock solution with differential nucleic acid binding activity that distinguishes double-stranded DNA from RNA, and it can be used for assays related to nucleic acid distribution and DNA localization. Methyl Green Staining Solution (Chloroform-Extracted, 1%) functions by competitively binding to the phosphate groups of nucleic acids and stably binds to intact double-stranded DNA; RNA within the oocyte-specific ribonucleoprotein complex specifically binds to this dye, and RNase completely blocks this binding reaction. The nucleic acid recognition of Methyl Green Staining Solution (Chloroform-Extracted, 1%) depends on the size of the dye cation and the intact double-helical structure of DNA^[1][2].