Mpro inhibitor N3 hemihydrate
Based on 1 publication(s) in Google Scholar
Mpro inhibitor N3 hemihydrate is a potent inhibitor of SARS-CoV-2 Mpro with an EC50 of 16.77 μM for SARS-CoV-2. Mpro inhibitor N3 hemihydrate specifically inhibits Mpro from multiple coronaviruses, including SARS-CoV and MERS-CoV. Mpro inhibitor N3 hemihydrate displays inhibition against HCoV-229E, FIPV, and MHV-A59 with individual IC50 of 4.0 μM, 8.8 μM, and 2.7 μM, respectively.
For research use only. We do not sell to patients.
- Purity: 99.86%
- Formula: C35H48N6O8·1/2H2O
- Molecular Weight:689.80
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Mpro inhibitor N3 hemihydrate
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Biological Activity
EC50: 16.77 μM (SARS-CoV-2)[1]
Chemical Information
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Appearance Solid
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Molecular Weight 689.80
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Formula C35H48N6O8·1/2H2O
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Color White to off-white
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SMILES
O=C([C@H](CC(C)C)NC([C@H](C(C)C)NC([C@H](C)NC(C1=NOC(C)=C1)=O)=O)=O)N[C@H](/C=C/C(OCC2=CC=CC=C2)=O)C[C@H](CCN3)C3=O.[1/2].O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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J Virol
Cleavage of TOM1 by the SARS-CoV-2 main protease NSP5 prevents autophagic degradation of viral envelope. [Abstract]2026 Jun 12:e0043426. PMID: 42283626
Solvent & Solubility
DMSO : 100 mg/mL (144.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Jin Z, et al. Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors. Nature. 2020;582(7811):289-293. [Content Brief]
[2]. Yang H, et al. Design of wide-spectrum inhibitors targeting coronavirus main proteases [published correction appears in PLoS Biol. 2005 Nov;3(11):e428]. PLoS Biol. 2005;3(10):e324. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4497 mL | 7.2485 mL | 14.4970 mL | 36.2424 mL |
| 5 mM | 0.2899 mL | 1.4497 mL | 2.8994 mL | 7.2485 mL | |
| 10 mM | 0.1450 mL | 0.7248 mL | 1.4497 mL | 3.6242 mL | |
| 15 mM | 0.0966 mL | 0.4832 mL | 0.9665 mL | 2.4162 mL | |
| 20 mM | 0.0725 mL | 0.3624 mL | 0.7248 mL | 1.8121 mL | |
| 25 mM | 0.0580 mL | 0.2899 mL | 0.5799 mL | 1.4497 mL | |
| 30 mM | 0.0483 mL | 0.2416 mL | 0.4832 mL | 1.2081 mL | |
| 40 mM | 0.0362 mL | 0.1812 mL | 0.3624 mL | 0.9061 mL | |
| 50 mM | 0.0290 mL | 0.1450 mL | 0.2899 mL | 0.7248 mL | |
| 60 mM | 0.0242 mL | 0.1208 mL | 0.2416 mL | 0.6040 mL | |
| 80 mM | 0.0181 mL | 0.0906 mL | 0.1812 mL | 0.4530 mL | |
| 100 mM | 0.0145 mL | 0.0725 mL | 0.1450 mL | 0.3624 mL |
Mpro inhibitor N3 hemihydrate Related Classifications
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