N-Benzoylcytidine

製品番号: HY-78574 純度: 99.50%: Data Sheet SDS COA 取扱説明書
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N-Benzoylcytidine is a modified cytidine analogue that can be phosphorylated by uracil-cytidine kinase (UCK1 and UCK2). N-Benzoylcytidine can be used to synthesize 2-OH protective groups for solid-phase RNA synthesis, as well as synthetic oligonucleotides for UV induction and targeted gene silencing in zebrafish embryos.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

N-Benzoylcytidine 構造式

CAS 番号 : 13089-48-0

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solid
5 g	$25	在庫あり	Estimated Time of Arrival: December 31
25 g	$50	在庫あり	Estimated Time of Arrival: December 31
50 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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純度とドキュメンテーション
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製品説明

分子量

347.33

分子式

C₁₆H₁₇N₃O₆

CAS 番号

13089-48-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1N([C@H]2[C@H](O)[C@H](O)[C@@H](CO)O2)C=CC(NC(C3=CC=CC=C3)=O)=N1

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (143.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8791 mL	14.3955 mL	28.7911 mL
5 mM	0.5758 mL	2.8791 mL	5.7582 mL
10 mM	0.2879 mL	1.4396 mL	2.8791 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.40 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (14.40 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.50%

Select Batch:

データシート (277 KB) SDS (392 KB)

COA (256 KB) HNMR (134 KB) LCMS (116 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Van Rompay AR, et al. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. Mol Pharmacol. 2001 May;59(5):1181-6. [Content Brief]

[2]. Semenyuk A, et al. Synthesis of RNA using 2 ‘-O-DTM protection. Journal of the American Chemical Society, 2006, 128(38): 12356-12357.

[3]. Shestopalov IA, et al. Light-controlled gene silencing in zebrafish embryos. Nat Chem Biol. 2007 Oct;3(10):650-1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8791 mL	14.3955 mL	28.7911 mL	71.9777 mL
	5 mM	0.5758 mL	2.8791 mL	5.7582 mL	14.3955 mL
	10 mM	0.2879 mL	1.4396 mL	2.8791 mL	7.1978 mL
	15 mM	0.1919 mL	0.9597 mL	1.9194 mL	4.7985 mL
	20 mM	0.1440 mL	0.7198 mL	1.4396 mL	3.5989 mL
	25 mM	0.1152 mL	0.5758 mL	1.1516 mL	2.8791 mL
	30 mM	0.0960 mL	0.4799 mL	0.9597 mL	2.3993 mL
	40 mM	0.0720 mL	0.3599 mL	0.7198 mL	1.7994 mL
	50 mM	0.0576 mL	0.2879 mL	0.5758 mL	1.4396 mL
	60 mM	0.0480 mL	0.2399 mL	0.4799 mL	1.1996 mL
	80 mM	0.0360 mL	0.1799 mL	0.3599 mL	0.8997 mL
	100 mM	0.0288 mL	0.1440 mL	0.2879 mL	0.7198 mL