NLS (PKKKRKV) (hydrochloride)
Based on 3 publication(s) in Google Scholar
NLS (PKKKRKV) hydrochloride is the hydrochloride form of NLS (PKKKRKV) (HY-P1876). NLS (PKKKRKV) is a nuclear localization signal (NLS) derived from the simian virus 40 large tumor antigen (SV40 large T antigen), that mediates binding of the karyophilic protein to importin α. NLS (PKKKRKV) can function as a method to enhance nuclear entry in the field of gene transfer research.
For research use only. We do not sell to patients.
- Purity: 99.39%
- Formula: C40H79ClN14O8
- Molecular Weight:919.60
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NLS (PKKKRKV) (hydrochloride)
MoreAll DNA/RNA Synthesis Isoforms
More
Biological Activity
Pre-incubation of NLS (20:1 mass ratio; 72 h) with pmaxGFP plasmid prior to the preparation of LNP-M formulations significantly reduces the transfection efficiency of LNP-M/pGFP in 293T cells, resulting in a final GFP-positive cell proportion of 17.7%[3].
Pre-incubation of NLS (80:1 mass ratio; 24 h post-transfection) with DSPC prior to the preparation of LNP-M formulations significantly enhances the transfection efficiency of LNP-M/pGFP in 293T cells, resulting in a GFP-positive cell ratio of 94.6% while increasing MFI[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 919.60
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Formula C40H79ClN14O8
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Color White to off-white
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Sequence
Pro-Lys-Lys-Lys-Arg-Lys-Val
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Sequence Shortening
PKKKRKV
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Mol Cancer
2025 Jan 13;24(1):12. PMID: 39806486 -
Mikrochim Acta
Nucleus delivery of doxorubicin by gadolinium-functionalized carbon dot drug delivery system for HCC imaging and therapy. [Abstract]2025 Aug 23;192(9):616. PMID: 40849365 -
Solvent & Solubility
H2O : 500 mg/mL (543.71 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (108.74 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Zanta MA, et al. Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus. Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):91-6. [Content Brief]
[2]. Cao S, et al. Enhanced effect of nuclear localization signal peptide during ultrasound‑targeted microbubble destruction‑mediated gene transfection. Mol Med Rep. 2017 Jul;16(1):565-572. [Content Brief]
[3]. Chai D, et al. Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity. Mol Cancer. 2025;24(1):12. Published 2025 Jan 13. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0874 mL | 5.4371 mL | 10.8743 mL | 27.1857 mL |
| 5 mM | 0.2175 mL | 1.0874 mL | 2.1749 mL | 5.4371 mL | |
| 10 mM | 0.1087 mL | 0.5437 mL | 1.0874 mL | 2.7186 mL | |
| 15 mM | 0.0725 mL | 0.3625 mL | 0.7250 mL | 1.8124 mL | |
| 20 mM | 0.0544 mL | 0.2719 mL | 0.5437 mL | 1.3593 mL | |
| 25 mM | 0.0435 mL | 0.2175 mL | 0.4350 mL | 1.0874 mL | |
| 30 mM | 0.0362 mL | 0.1812 mL | 0.3625 mL | 0.9062 mL | |
| 40 mM | 0.0272 mL | 0.1359 mL | 0.2719 mL | 0.6796 mL | |
| 50 mM | 0.0217 mL | 0.1087 mL | 0.2175 mL | 0.5437 mL | |
| 60 mM | 0.0181 mL | 0.0906 mL | 0.1812 mL | 0.4531 mL | |
| 80 mM | 0.0136 mL | 0.0680 mL | 0.1359 mL | 0.3398 mL | |
| 100 mM | 0.0109 mL | 0.0544 mL | 0.1087 mL | 0.2719 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NLS (PKKKRKV) (hydrochloride) Related Classifications
HY-P1876B Related Classifications
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