NLS (PKKKRKV)
Based on 3 publication(s) in Google Scholar
NLS (PKKKRKV) is a nuclear localization signal (NLS) derived from the SV40 large T antigen, which mediates the binding of karyophilic proteins to importin α. NLS (PKKKRKV) serves as a method to enhance nuclear entry in the field of gene transfer research.
For research use only. We do not sell to patients.
- Purity: 99.03%
- CAS No.: 95088-49-6
- Formula: C40H78N14O8
- Molecular Weight:883.14
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NLS (PKKKRKV)
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
Pre-incubation of NLS (20:1 mass ratio; 72 h) with pmaxGFP plasmid prior to the preparation of LNP-M formulations significantly reduces the transfection efficiency of LNP-M/pGFP in 293T cells, resulting in a final GFP-positive cell proportion of 17.7%[3].
Pre-incubation of NLS (80:1 mass ratio; 24 h post-transfection) with DSPC prior to the preparation of LNP-M formulations significantly enhances the transfection efficiency of LNP-M/pGFP in 293T cells, resulting in a GFP-positive cell ratio of 94.6% while increasing MFI[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 95088-49-6
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Appearance Solid
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Molecular Weight 883.14
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Formula C40H78N14O8
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Color White to off-white
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Sequence Shortening
PKKKRKV
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Mol Cancer
2025 Jan 13;24(1):12. PMID: 39806486 -
Mikrochim Acta
Nucleus delivery of doxorubicin by gadolinium-functionalized carbon dot drug delivery system for HCC imaging and therapy. [Abstract]2025 Aug 23;192(9):616. PMID: 40849365 -
Purity & Documentation
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Data Sheet (262 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Cao S, et al. Enhanced effect of nuclear localization signal peptide during ultrasound‑targeted microbubble destruction‑mediated gene transfection. Mol Med Rep. 2017;16(1):565-572. [Content Brief]
[2]. Zanta MA, et al. Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus. Proc Natl Acad Sci U S A. 1999;96(1):91-96. [Content Brief]
[3]. Chai D, et al. Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity. Mol Cancer. 2025;24(1):12. Published 2025 Jan 13. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NLS (PKKKRKV) Related Classifications
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