Phytosphingosine (Synonyms: 4-Hydroxysphinganine)

Name: Phytosphingosine
Brand: MedChemExpress
SKU: HY-W011303
Rating: 5.0 (4 reviews)

Cat. No.: HY-W011303 Purity: 99.85%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Phytosphingosine is a phospholipid with anti-inflammatory, antibacterial, and anti-cancer activities, which can induce apoptosis. Phytosphingosine is an immune regulator and can be used in the study of inflammatory skin diseases. Phytosphingosine is also an activator of GPR120 with an IC₅₀ value of 33.4 μM and can be used in the study of type II diabetes.

For research use only. We do not sell to patients.

CAS No. : 554-62-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Phytosphingosine:

Phytosphingosine-d₇ Get quote
Phytosphingosine (Standard) Get quote

4 Publications Citing Use of MCE Phytosphingosine

ELISA

Histological Imaging/Staining

Cell Imaging/Staining

: Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]; Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly decreased the levels of IL-6, KC, and IL-1β in CS-exposed mice.

: Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]; Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) reduced infiltration of inflammatory cells around the airways in CS-exposed mice and inflammation scores.

: Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]; Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly decreased total and various inflammatory cell counts, particularly macrophages, in bronchoalveolar lavage fluid (BALF) of CS-exposed mice.

: Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]; Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly downregulated p53, p21, and p-Rb in CS-exposed mice.

: Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]; Co-immunofluorescence targeting p53 and p21 revealed that PHS treatment reduced the expression of senescent proteins in the airway epithelium of CS-exposed mice.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	IC₅₀	3.75 μM Compound: Phytosphingosine	Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	4.12 μM Compound: Phytosphingosine	Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	5.29 μM Compound: Phytosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	6.57 μM Compound: Phytosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]

In Vitro

Phytosphingosine (200-400 μg/mL; 60-240 min) can inhibit the growth of Gram-positive and Gram-negative bacteria, yeast, and molds, and significantly inhibit the release of IL-1a, exhibiting anti-inflammatory activity^[1].
Phytosphingosine (1-5 μg/mL; 24-48 h) inhibits lung adenoma cell proliferation by inducing G2/M phase arrest, apoptosis and mitochondria-dependent pathway cell death in A549 and LLC cells, with IC₅₀ values of 4.3 μg/mL and 4.5μg/mL, respectively^[2].
Phytosphingosine (80 μM; 1-10 days) has antibacterial activity and can inhibit the growth ofS. sclerotiorum, S. indica and V. longisporum^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	A549, LLC, BEAS-2B
Concentration:	1 μg/mL, 2 μg/mL, 3 μg/mL, 4 μg/mL, 5 μg/mL
Incubation Time:	24 h, 48 h
Result:	Significantly inhibited cell proliferation in a concentration- and time-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	A549
Concentration:	1 μg/mL, 3 μg/mL, 5 μg/mL
Incubation Time:	24 h
Result:	Significantly increased the proportion of G2/M phase cells.

Western Blot Analysis^[2]

Cell Line:	A549
Concentration:	1 μg/mL, 3 μg/mL, 5 μg/mL
Incubation Time:	24 h
Result:	Up-regulated the pro-apoptotic factor Bax protein level and down-regulated the anti-apoptotic factor Bcl-2 protein level. Promoted the release of cytochrome c to up-regulate caspase 9 and caspase 3 levels, leading to PARP cleavage.

In Vivo

Phytosphingosine (0.5-2 mg/kg; Intraperitoneal injection; once every other day for 10 times) has anti-tumor activity in C57BL/6 mice with lung cancer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

317.51

Formula

C₁₈H₃₉NO₃

CAS No.

554-62-1

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCC[C@@H](O)[C@@H](O)[C@@H](N)CO

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (15.75 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1495 mL	15.7475 mL	31.4951 mL
5 mM	0.6299 mL	3.1495 mL	6.2990 mL
10 mM	0.3150 mL	1.5748 mL	3.1495 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 3.33 mg/mL (10.49 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (240 KB) HNMR (264 KB) RP-HPLC (245 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. 1. Pavicic T, Wollenweber U, Farwick M, et al. Anti‐microbial and‐inflammatory activity and efficacy of phytosphingosine: an in vitro and in vivo study addressing acne vulgaris[J]. International journal of cosmetic science, 2007, 29(3): 181-190. [Content Brief]

[2]. Cai Q, et al. Phytosphingosine inhibits the growth of lung adenocarcinoma cells by inducing G2/M-phase arrest, apoptosis, and mitochondria-dependent pathway cell death in vitro and in vivo. Chem Biol Interact. 2024 Jan 5;387:110795. [Content Brief]

[3]. Glenz R, et al. The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens. Sci Rep. 2022 Jan 20;12(1):1081. [Content Brief]

[4]. Nagasawa T, et al. Phytosphingosine is a novel activator of GPR120. J Biochem. 2018 Jul 1;164(1):27-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1495 mL	15.7475 mL	31.4951 mL	78.7377 mL
	5 mM	0.6299 mL	3.1495 mL	6.2990 mL	15.7475 mL
	10 mM	0.3150 mL	1.5748 mL	3.1495 mL	7.8738 mL
	15 mM	0.2100 mL	1.0498 mL	2.0997 mL	5.2492 mL

Phytosphingosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phytosphingosine (Synonyms: 4-Hydroxysphinganine)

Customer Review

Other Forms of Phytosphingosine:

Phytosphingosine Related Antibodies

4 Publications Citing Use of MCE Phytosphingosine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Free Fatty Acid Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Phytosphingosine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phytosphingosine Related Classifications

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Customer Review

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