D-Erythro-dihydrosphingosine
Based on 1 publication(s) in Google Scholar
D-Erythro-dihydrosphingosin directly inhibits cytosolic phospholipase A2α (cPLA2α) activity.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 764-22-7
- Formula: C18H39NO2
- Molecular Weight:301.51
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) D-Erythro-dihydrosphingosine
MoreAll Endogenous Metabolite Isoforms
MoreAll Phospholipase Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| FL5.12 | IC50 |
3.5 μM
Compound: 7
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Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis
Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis
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[PMID: 27475534] |
| HBL-100 | GI50 |
25 μM
Compound: 10d
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Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay
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[PMID: 25899335] |
| HeLa | GI50 |
15 μM
Compound: 10d
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Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay
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[PMID: 25899335] |
| Jurkat | IC50 |
3.53 μM
Compound: Dihydrosphingosine
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Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay
Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay
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[PMID: 17400555] |
| Jurkat | IC50 |
3.68 μM
Compound: Dihydrosphingosine
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Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay
Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay
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[PMID: 17400555] |
| Jurkat | IC50 |
4.9 μM
Compound: Dihydrosphingosine
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Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay
Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay
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[PMID: 17400555] |
| Jurkat | IC50 |
6.31 μM
Compound: Dihydrosphingosine
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Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay
Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay
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[PMID: 17400555] |
| SW1573 | GI50 |
4.5 μM
Compound: 10d
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Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay
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[PMID: 25899335] |
| T47D | GI50 |
22 μM
Compound: 10d
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Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay
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[PMID: 25899335] |
| WiDr | GI50 |
19 μM
Compound: 10d
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Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay
Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay
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[PMID: 25899335] |
D-Erythro-dihydrosphingosine (D-erythro-DHS) significantly inhibits mastoparan-, but not Na3VO4-, stimulated arachidonic acid release in PC12 cells. D-Erythro-dihydrosphingosine at 100 AM slightly inhibits [3H]Arachidonic acid release, and 5 μM Ionomycin alone stimulates the release significantly. In the presence of 5 μM Ionomycin, 100 μM C2-ceramide stimulates the release, but D-Erythro-dihydrosphingosine (100 μM) inhibits the release. D-Erythro-dihydrosphingosine inhibits arachidonic acid release in cells and/or cPLA2α activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 764-22-7
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Appearance Solid
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Molecular Weight 301.51
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Formula C18H39NO2
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Color White to off-white
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SMILES
CCCCCCCCCCCCCCC[C@@H](O)[C@@H](N)CO
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Synonyms
Sphinganine (d18:0)
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Host Microbe
Gut DNA virome enterotype dictates inflammation heterogeneity through tuning the phage-bacteria-sphingosine-intestine axis in Crohn's disease. [Abstract]2026 Mar 11;34(3):460-478.e9. PMID: 41666919
Solvent & Solubility
Ethanol : 50 mg/mL (165.83 mM; Need ultrasonic)
DMSO : 10 mg/mL (33.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (3.32 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (3.32 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 3.3166 mL | 16.5832 mL | 33.1664 mL | 82.9160 mL |
| 5 mM | 0.6633 mL | 3.3166 mL | 6.6333 mL | 16.5832 mL | |
| 10 mM | 0.3317 mL | 1.6583 mL | 3.3166 mL | 8.2916 mL | |
| 15 mM | 0.2211 mL | 1.1055 mL | 2.2111 mL | 5.5277 mL | |
| 20 mM | 0.1658 mL | 0.8292 mL | 1.6583 mL | 4.1458 mL | |
| 25 mM | 0.1327 mL | 0.6633 mL | 1.3267 mL | 3.3166 mL | |
| 30 mM | 0.1106 mL | 0.5528 mL | 1.1055 mL | 2.7639 mL | |
| Ethanol | 40 mM | 0.0829 mL | 0.4146 mL | 0.8292 mL | 2.0729 mL |
| 50 mM | 0.0663 mL | 0.3317 mL | 0.6633 mL | 1.6583 mL | |
| 60 mM | 0.0553 mL | 0.2764 mL | 0.5528 mL | 1.3819 mL | |
| 80 mM | 0.0415 mL | 0.2073 mL | 0.4146 mL | 1.0364 mL | |
| 100 mM | 0.0332 mL | 0.1658 mL | 0.3317 mL | 0.8292 mL |
D-Erythro-dihydrosphingosine Related Classifications
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