Role of the 12-lipoxygenase pathway in diabetes pathogenesis and complications

  • Pharmacol Ther. 2019 Mar:195:100-110. doi: 10.1016/j.pharmthera.2018.10.010.
A D Dobrian  1 M A Morris  2 D A Taylor-Fishwick  3 T R Holman  4 Y Imai  5 R G Mirmira  6 J L Nadler  7
Affiliations
  • 1. Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA, United States.
  • 2. Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, VA, United States.
  • 3. Department of Microbiology, Cell and Molecular Biology, Eastern Virginia Medical School, Norfolk, VA, United States.
  • 4. Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA, United States.
  • 5. University of Iowa Carver College of Medicine, Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa, city, IA, United States.
  • 6. Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • 7. Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, VA, United States. Electronic address: [email protected].
Abstract

12-lipoxygenase (12-LOX) is one of several enzyme isoforms responsible for the metabolism of arachidonic acid and Other poly-unsaturated fatty acids to both pro- and anti-inflammatory lipid mediators. Mounting evidence has shown that 12-LOX plays a critical role in the modulation of inflammation at multiple checkpoints during diabetes development. Due to this, interventions to limit pro-inflammatory 12-LOX metabolites either by isoform-specific 12-LOX inhibition, or by providing specific fatty acid substrates via dietary intervention, has the potential to significantly and positively impact health outcomes of patients living with both type 1 and type 2 diabetes. To date, the development of truly specific and efficacious inhibitors has been hampered by homology of LOX family members; however, improvements in high throughput screening have improved the inhibitor landscape. Here, we describe the function and role of human 12-LOX, and mouse 12-LOX and 12/15-LOX, in the development of diabetes and diabetes-related complications, and describe promise in the development of strategies to limit pro-inflammatory metabolites, primarily via new small molecule 12-LOX inhibitors.

Keywords
Inflammation; Lipoxygenase; Lipoxygenase inhibitors; Type 1 diabetes; Type 2 diabetes-related complications.