Syringin protects against colitis by ameliorating inflammation

  • Arch Biochem Biophys. 2020 Feb 15;680:108242. doi: 10.1016/j.abb.2019.108242.
Haihua Zhang  1 ,  Haijun Gu  2 ,  Qinghui Jia  1 ,  Yanqing Zhao  3 ,  Hongqiang Li  1 ,  Shurui Shen  1 ,  Xin Liu  1 ,  Guisheng Wang  4 ,  Qiumei Shi  5
Affiliations
  • 1. College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, 066004, People's Republic of China.
  • 2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, 130112, People's Republic of China.
  • 3. Wuqiao Bureau of Agriculture and Rural Affairs, Cangzhou, Hebei, 061899, People's Republic of China.
  • 4. Animal Disease Prevention and Control Center of Shandong Province, Jinan, Shandong, 250022, People's Republic of China.
  • 5. College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, 066004, People's Republic of China. Electronic address: [email protected].
PMID: 31899146 DOI: 10.1016/j.abb.2019.108242
Abstract

Inflammatory bowel disease (IBD) is a chronic inflammatory condition with high incidence. Syringin exhibits multiple pharmacological properties, including anti-inflammatory effects. However, the effect of syringin on inflammation of IBD is still unclear. Here, the dextran sulfate sodium (DSS)-induced colitis model was established in vivo. Rat intestinal epithelium IEC6 cells were treated with lipopolysaccharide (LPS) in vitro. Syringin inhibited DSS or LPS-induced overproduction of proinflammatory cytokines (IL-1β, IL-6, TNF-α) and proinflammatory substances (iNOS, COX-2). Moreover, syringin inactivated the proinflammatory NF-κB p65 pathway by decreasing IκBα phosphorylation at Ser 32. The activation of antioxidant Nrf2 signaling pathway was promoted by syringin. Additionally, LPS-induced inflammation in IEC6 cells was also suppressed by NF-κB Inhibitor PDTC and Nrf2 activator RTA408. The anti-inflammatory effects of syringin were comparable to these two reagents. Taken together, our results suggest that syringin shows protective effects on intestinal inflammation through inhibiting NF-κB, while activating Nrf2 signaling pathway in colitis.

Keywords
Colitis; Inflammation; NF-κB pathway; Nrf2 pathway; Syringin.
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