ARMET/MANF Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
ARMET/MANF proteins regulate the survival of dopaminergic neurons in the ventral midbrain and modulate GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances GABA inhibitory currents and inhibits cell proliferation while preventing ER stress-induced cell death. ARMET/MANF Protein, Mouse (HEK293, His) is the recombinant mouse-derived ARMET/MANF protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
ARMET/MANF proteins regulate the survival of dopaminergic neurons in the ventral midbrain and modulate GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances GABA inhibitory currents and inhibits cell proliferation while preventing ER stress-induced cell death. ARMET/MANF Protein, Mouse (HEK293, His) is the recombinant mouse-derived ARMET/MANF protein, expressed by HEK293 , with C-His labeled tag.
Background
ARMET/MANF protein is a vital regulator of dopaminergic neuron survival in the ventral mid-brain, while also modulating GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances both spontaneous and evoked GABAergic inhibitory postsynaptic currents in these neurons. Additionally, it inhibits cell proliferation and protects against cell death induced by ER stress. In normal conditions, ARMET/MANF protein is retained in the ER/sarcoplasmic reticulum through its association with the chaperone protein HSPA5. However, it is upregulated and secreted by the ER/SR in response to ER stress and hypoxia. Upon secretion, it directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide on the outer cell membrane of target cells, facilitating its uptake through endocytosis. This sulfatide binding is crucial for ARMET/MANF's role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. ARMET/MANF protein also interacts with HSPA5 and is a component of a complex that includes CRELD2, MATN3, and PDIA4.
Verified Bioactivity
Measured in a cell proliferation assay using rat C6 cells. The ED50 for this effect is 1-3 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q9CXI5/NP_083379.2 (L22-L179)
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Molecular Construction
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N-term
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ARMET (L22-L179)
Accession # Q9CXI5/NP_083379.2 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
MANF; Arginine-Rich, Mutated In Early Stage Tumors; Prev. ARMET; Protein ARMET; ARP; DDDS; Mesencephalic Astrocyte-Derived Neurotrophic Factor; Mesencephalic Astrocyte Derived Neurotrophic Factor; Arginine-Rich Protein
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AA Sequence
LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGEMCKGCAEKSDYIRKINELMPKYAPKAASARTDL
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Predicted Molecular Mass
19.6 kDa
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Molecular Weight
Approximately 21 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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