BACE1 Protein, Human (HEK293, His)

The BACE1 protein is an important member of the aspartic protease family and is critical in Alzheimer's disease pathology. Alternative splicing generates multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to produce the mature protease. BACE1 Protein, Human (HEK293, His) is the recombinant human-derived BACE1 protein, expressed by HEK293 , with C-10*His labeled tag.

BACE1, a member of the peptidase A1 family of aspartic proteases, plays a pivotal role in Alzheimer's disease pathology. Through alternative splicing, this gene generates multiple transcript variants, with at least one encoding a preproprotein that undergoes proteolytic processing to yield the mature protease. As a transmembrane protease, BACE1 catalyzes the initial step in the production of amyloid beta peptides from the amyloid precursor protein. Amyloid beta peptides constitute the primary component of the amyloid beta plaques that accumulate in the brains of individuals with Alzheimer's disease. The expression of BACE1 is widespread, showing ubiquity across various tissues, including the pancreas, brain, and 24 other tissues, underscoring its significance in diverse physiological contexts.

Human

HEK293

C-10*His

NP_036236.1 (T22-T457)

23621  [NCBI]

Partial

TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMT

Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.