BAFF/TNFSF13B Protein, Human (CHO)

BAFF/TNFSF13B Protein, Human (CHO) is a member of the tumor necrosis factor (TNF) superfamily, and plays an essential role in peripheral B-cell homeostasis.

B-cell activating factor (BAFF), a member of the tumor necrosis factor (TNF) superfamily, is secreted by various myeloid cells such as macrophages, dendritic cells, and neutrophils. BAFF is a potent survival factor for B cells and plays an essential role in peripheral B-cell homeostasis^[1].

1.The ED₅₀ is <20 ng/mL as measured by human RPMI 8226 cells, corresponding to a specific activity of >5.0 × 10⁴ units/mg.
2.Measured by its binding ability in a functional ELISA.When Recombinant BCMA Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human BAFF. The ED₅₀ for this effect is <4 ng/mL.

• 
  Measured by its binding ability in a functional ELISA.When Recombinant BCMA Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human BAFF binds with an ED₅₀ of 3.639 ng/mL.

Human

CHO

Tag Free

Q9Y275-1 (A134-L285)

10673  [NCBI]

Full Length of TNFSF13B soluble form

AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Approximately 18 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Motobayashi M, et al. An increase in circulating B cell-activating factor in childhood-onset ocular myasthenia gravis. Pediatr Neurol. 2015 Apr;52(4):404-9.  [Content Brief]