Copy Link Print Information

BAFF/TNFSF13B Protein, Human (CHO)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

BAFF/TNFSF13B Protein, Human (CHO) is a member of the tumor necrosis factor (TNF) superfamily, and plays an essential role in peripheral B-cell homeostasis.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • BAFF/TNFSF13B Protein, Human (CHO) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

BAFF/TNFSF13B Protein, Human (CHO) is a member of the tumor necrosis factor (TNF) superfamily, and plays an essential role in peripheral B-cell homeostasis.

Background

B-cell activating factor (BAFF), a member of the tumor necrosis factor (TNF) superfamily, is secreted by various myeloid cells such as macrophages, dendritic cells, and neutrophils. BAFF is a potent survival factor for B cells and plays an essential role in peripheral B-cell homeostasis[1].

Verified Bioactivity

1.The ED50 is <20 ng/mL as measured by human RPMI 8226 cells, corresponding to a specific activity of >5.0 × 104 units/mg.
2.Measured by its binding ability in a functional ELISA.When Recombinant BCMA Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human BAFF. The ED50 for this effect is <4 ng/mL.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag Tag Free
  • Accession

    Q9Y275-1 (A134-L285)

  • Gene ID
    10673  [NCBI]
  • Molecular Construction
    • N-term
    • BAFF (A134-L285)
      Accession # Q9Y275-1
    • C-term
  • Protein Length

    Full Length of TNFSF13B, soluble form

  • Synonyms

    TNFSF13B; Dendritic Cell-Derived TNF-Like Molecule; Prev. TNFSF20; Tumor Necrosis Factor Ligand 7A; TALL-1; ZTNF4; TALL1; Tumor Necrosis Factor (Ligand) Superfamily, Member 20; BAFF; TNF- And APOL-Related Leukocyte Expressed Ligand 1; BLYS; Tumor Necrosis

  • AA Sequence

    AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

  • Predicted Molecular Mass

    17 kDa

  • Molecular Weight

    Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Motobayashi M, et al. An increase in circulating B cell-activating factor in childhood-onset ocular myasthenia gravis. Pediatr Neurol. 2015 Apr;52(4):404-9. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA.When Recombinant BCMA Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Human BAFF binds with an ED50 of 3.639 ng/mL.

  • 1/16

    BAFF/TNFSF13B Protein, Human (CHO) purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Feb 11;1870(4):167066. [Abstract]

    Customer Validation
     The incubation of BAFF (BAFF/TNFSF13B) (100ng/mL) cytokine for 24 h increased the transcriptional levels of LRRC8A in HCT116 cells (n = 6).

  • BAFF/TNFSF13B Protein, Human (CHO) Product Image
    1/16
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • Experimental Validation Results for BAFF/TNFSF13B Protein, Human (CHO)
  • BAFF/TNFSF13B Protein, Human (CHO) Product Image