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  5. CD24 Protein, Mouse (HEK293, hFc)

CD24 Protein, Mouse (HEK293, hFc)

Cat. No.: HY-P72747
COA Handling Instructions

The CD24 protein may coordinate cellular differentiation of various cell types, particularly in early thymocyte development. Activation of its signaling involves binding of a lectin-like ligand to the CD24 carbohydrate, triggering the release of second messengers from GPI anchors. CD24 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived CD24 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $55 In-stock
50 μg $160 In-stock
100 μg $270 Get quote
500 μg $1150 Get quote
1 mg $1800 In-stock
> 1 mg   Get quote  

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  • Biological Activity

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Description

The CD24 protein may coordinate cellular differentiation of various cell types, particularly in early thymocyte development. Activation of its signaling involves binding of a lectin-like ligand to the CD24 carbohydrate, triggering the release of second messengers from GPI anchors. CD24 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived CD24 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

CD24 Protein is postulated to play a pivotal role in the cell differentiation processes of various cell types, with a specific implication in early thymocyte development. Its signaling potential is suggested to be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. Additionally, CD24 is implicated in modulating B-cell activation responses, indicative of its regulatory influence in immune processes. In conjunction with SIGLEC10, CD24 may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. This CD24:SIGLEC10 complex is proposed to inhibit HMGB1-mediated tissue damage immune responses, suggesting CD24's role in immune regulation and control of autoimmunity. The multifaceted functions of CD24 underscore its potential as a key player in diverse cellular and immune processes.

Biological Activity

Immobilized Mouse CD24 at 10 μg/mL (100 μL/well) can bind Anti-CD24 Antibody, The ED50 for this effect is 0.1823 μg/mL, corresponding to a specific activity is 5.485×10^3 units/mg.

  • Immobilized Mouse CD24 at 10 μg/mL (100 μL/well) can bind Anti-CD24 Antibody, The ED50 for this effect is 0.1823 μg/mL, corresponding to a specific activity is 5.485×10^3 units/mg.
Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P24807 (N27-G53)

Gene ID
Molecular Construction
N-term
CD24 (N27-G53)
Accession # P24807
hFc
C-term
Synonyms
Signal transducer CD24; Ly-52; HAS; Nectadrin; R13-Ag; CD24; CD24A
AA Sequence

NQTSVAPFPGNQNISASPNPSNATTRG

Molecular Weight

39-56 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD24 Protein, Mouse (HEK293, hFc)
Cat. No.:
HY-P72747
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