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  5. CD98 Protein, Human (HEK293, His)

CD98 Protein, Human (HEK293, His)

Cat. No.: HY-P75368
COA Handling Instructions

The CD98 protein acts as a chaperone, promoting the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters and affects the substrate specificity of amino acid antiporters. CD98 Protein, Human (HEK293, His) is the recombinant human-derived CD98 protein, expressed by HEK293 , with N-His labeled tag. The total length of CD98 Protein, Human (HEK293, His) is 425 a.a., with molecular weight of 60-80 kDa.

For research use only. We do not sell to patients.

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Description

The CD98 protein acts as a chaperone, promoting the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters and affects the substrate specificity of amino acid antiporters. CD98 Protein, Human (HEK293, His) is the recombinant human-derived CD98 protein, expressed by HEK293 , with N-His labeled tag. The total length of CD98 Protein, Human (HEK293, His) is 425 a.a., with molecular weight of 60-80 kDa.

Background

CD98 protein functions as a chaperone that aids in the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters, including SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10, and SLC7A11, acting as amino acid antiporters with substrate specificity determined by the SLC7A subunit. For instance, the heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate. Additionally, SLC3A2/SLC7A5, when associated with LAPTM4B, is recruited to lysosomes, promoting leucine uptake and mTORC1 activation. CD98 is essential for plasma membrane localization, stability, and transport activity of various SLC7 transporters. Moreover, it plays a pivotal role in modulating integrin-related signaling, contributing to cell spreading, migration, and tumor progression. In the context of hepatitis C virus (HCV) infection, the SLC3A2 and SLC7A5/LAT1 complex facilitates viral entry into host cells and increases L-leucine uptake, thereby enhancing mTORC1 signaling activation and contributing to HCV-mediated pathogenesis.

Biological Activity

1.Immobilized CD98, Human His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-CD98 Antibody, mFc Tag with the EC50 of 11.1 ng/mL determined by ELISA.
2.Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody. The ED50 for this effect is 0.03379 nM.

  • Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody, The ED50 for this effect is 0.03379 nM.
Species

Human

Source

HEK293

Tag

N-His

Accession

P08195-1 (R206-A630)

Gene ID
Molecular Construction
N-term
His
CD98 (R206-A630)
Accession # P08195-1
C-term
Synonyms
4F2 cell-surface antigen heavy chain; 4F2hc; CD98; SLC3A2
AA Sequence

RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

Molecular Weight

60-80 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD98 Protein, Human (HEK293, His)
Cat. No.:
HY-P75368
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