CES2/carboxylesterase 2 Protein, Human (HEK293, His, solution)

The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293, His, solution) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-10*His labeled tag.

The CES2/Carboxylesterase 2 Protein plays a crucial role in the detoxification of xenobiotics and the activation of ester and amide prodrugs. It exhibits high catalytic efficiency in the hydrolysis of various compounds, including cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 hydrolyzes aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. Notably, it is involved in the conversion of monoacylglycerides to free fatty acids and glycerol. Furthermore, CES2 demonstrates activity in the hydrolysis of 2-arachidonoylglycerol and prostaglandins, highlighting its versatility in enzymatic functions with potential implications in various biological processes and drug metabolism.

Measured by its ability to hydrolyze p-nitrophenylacetate.Which can be measured by absorbance at 410 nm. The specific activity is >20000 pmol/min/µg.

Human

HEK293

C-10*His

O00748-1 (Q27-L559)

8824

Full Length of Isoform-1 Mature Protein

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Approximately 50-70 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM NaAc, 150 mM NaCl, 10% Glycerol, pH 5.5.

<1 EU/μg, determined by LAL method

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.