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CES2/carboxylesterase 2 Protein, Human (HEK293, His)

The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293, His) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CES2/carboxylesterase 2 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293, His) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag.

Background

The CES2/Carboxylesterase 2 Protein plays a crucial role in the detoxification of xenobiotics and the activation of ester and amide prodrugs. It exhibits high catalytic efficiency in the hydrolysis of various compounds, including cocaine, 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. Additionally, CES2 hydrolyzes aspirin, substrates with large alcohol groups and small acyl groups, as well as endogenous lipids such as triacylglycerol. Notably, it is involved in the conversion of monoacylglycerides to free fatty acids and glycerol. Furthermore, CES2 demonstrates activity in the hydrolysis of 2-arachidonoylglycerol and prostaglandins, highlighting its versatility in enzymatic functions with potential implications in various biological processes and drug metabolism.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    O00748-1 (Q27-L559)

  • Gene ID
    8824  [NCBI]
  • Molecular Construction
    • N-term
    • CES2 (Q27-L559)
      Accession # O00748-1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CES2; Carboxylesterase 2 (Intestine, Liver); Carboxylesterase 2; Cocaine Esterase; CE-2; Intestinal Carboxylesterase; Liver Carboxylesterase-2; ICE; Carboxylic Ester Hydrolase; CES2A1; PCE-2; Methylumbelliferyl-Acetate Deacetylase 2; HCE-2

  • AA Sequence

    QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

  • Predicted Molecular Mass

    60.4 kDa

  • Molecular Weight

    Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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