CRBN-DDB1 Protein, Human (Biotinylated, sf9, Avi)

The DDB1-CRBN complex is a crucial component of the E3 ubiquitin ligase system. CRBN-DDB1 is widely used in targeted protein degradation (TPD) and oncology research, it is essential for studying the mechanism of immunomodulatory drugs (IMiDs) and discovering novel PROTACs. CRBN-DDB1 Protein, Human (Biotinylated, sf9, Avi) is a recombinant protein expressed by Sf9 insect cells , with Avi labeled tag^[1][2][3][4].

CRBN (Cereblon) and DDB1 (DNA damage-binding protein 1) are proteins that play crucial roles in various cellular processes, including DNA repair, cell cycle regulation, and protein degradation. CRBN is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins. Binding of CRBN to CUL4 is mediated in part by DDB1, a component of multiple DCX class ligases. This complex plays a critical role in the ubiquitin proteasome pathway, which controls the targeted degradation of specific proteins. Understanding the intricate roles of CRBN and DDB1 in cellular processes is crucial for deciphering their potential as therapeutic targets and advancing knowledge of various diseases, particularly cancer, where dysregulation of these proteins has been observed^[1][2][3][4].

CRBN-DDB1 Protein, Human (Biotinylated, sf9, Avi) exhibits similar apparent binding affinities for Lenalidomide (HY-A0003) and Pomalidomide (HY-10984) in fluorescent thermal melt assays, consistent with the findings in myeloma cell lysates^[1].
CRBN-DDB1 Protein, Human (Biotinylated, sf9, Avi) forms a CRBN:MGD:CRBN ternary complex with MRT-31619 (HY-180538)^[2].

Human

Sf9 insect cells

N-Avi

Q96SW2-1 (M1-L442)&Q16531-1 (M1-H1140)

51185&1642

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

52.6 KDa&126.9 KDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

1.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
2.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Lopez-Girona A, et al. Cereblon is a direct protein target for immunomodulatory and antiproliferative activities of lenalidomide and pomalidomide. Leukemia. 2012 Nov;26(11):2326-35.  [Content Brief]

  [2]. Langousis G, et al. A degron-mimicking molecular glue drives CRBN homo-dimerization and degradation. Nat Commun. 2025 Nov 19;16(1):10157.  [Content Brief]

  [3]. Oleinikovas V, et al. From Thalidomide to Rational Molecular Glue Design for Targeted Protein Degradation. Annu Rev Pharmacol Toxicol. 2024 Jan 23;64:291-312.

  [4]. Chen Y, et al. Phosphorylation regulates cullin-based ubiquitination in tumorigenesis. Acta Pharm Sin B. 2021 Feb;11(2):309-321.