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DDR2 Protein, Human (HEK293, His)

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The DDR2 protein is a tyrosine kinase critical in tissue remodeling, acting as a cell surface receptor for fibrillar collagen and affecting cell differentiation, extracellular matrix remodeling, migration, and proliferation. DDR2 is critical for bone development and centrally regulates osteoblast differentiation and chondrocyte maturation through MAP kinase signaling and activation of RUNX2. DDR2 Protein, Human (HEK293, His) is the recombinant human-derived DDR2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The DDR2 protein is a tyrosine kinase critical in tissue remodeling, acting as a cell surface receptor for fibrillar collagen and affecting cell differentiation, extracellular matrix remodeling, migration, and proliferation. DDR2 is critical for bone development and centrally regulates osteoblast differentiation and chondrocyte maturation through MAP kinase signaling and activation of RUNX2. DDR2 Protein, Human (HEK293, His) is the recombinant human-derived DDR2 protein, expressed by HEK293 , with C-His labeled tag.

Background

DDR2 protein, a tyrosine kinase integral to the regulation of tissue remodeling, functions as a cell surface receptor for fibrillar collagen, exerting control over cell differentiation, extracellular matrix remodeling, cell migration, and proliferation. Crucial for normal bone development, DDR2 plays a central role in osteoblast differentiation and chondrocyte maturation through a signaling pathway involving MAP kinases, culminating in the activation of the transcription factor RUNX2. Additionally, DDR2 regulates extracellular matrix remodeling by up-regulating collagenases MMP1, MMP2, and MMP13, thereby facilitating cell migration and tumor cell invasion. Its involvement extends to promoting fibroblast migration and proliferation, contributing to the dynamic process of cutaneous wound healing.

Verified Bioactivity

This product does not contain protein kinase domain.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q16832/NP_001014796.1 (K22-R399)

  • Gene ID
    4921  [NCBI]
  • Molecular Construction
    • N-term
    • DDR2 (K22-R399)
      Accession # Q16832/NP_001014796.1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    DDR2; Discoidin Domain Receptor 2; Prev. TYRO10; Neurotrophic Tyrosine Kinase Receptor Related 3; Prev. NTRKR3; Migration-Inducing Gene 16 Protein; TKT; Cell Migration-Inducing Protein 20; Discoidin Domain-Containing Receptor Tyrosine Kinase 2; Hydroxyaryl-Protein Kinase; Discoidin Domain Receptor Family, Member 2; CD167b Antigen; Discoidin Domain-Containing Receptor 2; MIG20a; Receptor Protein-Tyrosine Kinase TKT; DDR2-N; CD167 Antigen-Like Family Member B; WRCN; Tyrosine-Protein Kinase TYRO10; Discoidin Domain Receptor Tyrosine Kinase 2; Neurotrophic Tyrosine Kinase, Receptor-Related 3

  • AA Sequence

    KAQVNPAICRYPLGMSGGQIPDEDITASSQWSESTAAKYGRLDSEEGDGAWCPEIPVEPDDLKEFLQIDLHTLHFITLVGTQGRHAGGHGIEFAPMYKINYSRDGTRWISWRNRHGKQVLDGNSNPYDIFLKDLEPPIVARFVRFIPVTDHSMNVCMRVELYGCVWLDGLVSYNAPAGQQFVLPGGSIIYLNDSVYDGAVGYSMTEGLGQLTDGVSGLDDFTQTHEYHVWPGYDYVGWRNESATNGYIEIMFEFDRIRNFTTMKVHCNNMFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQSDAAMYNNSEALPTSPMAPTTYDPMLKVDDSNTR

  • Predicted Molecular Mass

    44.1 kDa

  • Molecular Weight

    Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, PH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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