FGFR-1 Protein, Mouse (HEK293, Fc)

The FGFR-1 protein is a tyrosine-protein kinase receptor for fibroblast growth factors that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived FGFR-1 protein, expressed by HEK293 , with C-hFc labeled tag.

FGFR-1 protein, a tyrosine-protein kinase, serves as a cell-surface receptor for fibroblast growth factors and plays a crucial role in the intricate regulation of embryonic development, cell proliferation, differentiation, and migration. It is indispensable for normal mesoderm patterning, correct axial organization during embryonic development, skeletogenesis, and the development of the gonadotropin-releasing hormone (GnRH) neuronal system. Upon ligand binding, FGFR-1 activates multiple signaling cascades, phosphorylating key proteins such as PLCG1, FRS2, GAB1, and SHB. This activation leads to the production of signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate through PLCG1. Moreover, phosphorylation of FRS2 triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, mediating the activation of RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. FGFR1 also promotes the phosphorylation of SHC1, STAT1, and PTPN11/SHP2. Within the nucleus, it enhances the activity of RPS6KA1 and CREB1, contributing to the regulation of transcription. The down-regulation of FGFR1 signaling occurs through IL17RD/SEF and FGFR1 ubiquitination, internalization, and degradation.

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1).

Mouse

HEK293

C-hFc

P16092-1 (M1-E376)

14182  [NCBI]

Extracellular Domain

MWGWKCLLFWAVLVTATLCTARPAPTLPEQAQPWGVPVEVESLLVHPGDLLQLRCRLRDDVQSINWLRDGVQLVESNRTRITGEEVEVRDSIPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRRPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE

Approximately 100-110 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.