Frizzled-5 Protein, Human (HEK293, His)

Frizzled-5 Protein functions as a receptor for Wnt proteins, including WNT2, WNT10B, and WNT5A, although it does not activate WNT2B or WNT4 in vitro, and the in vivo context may vary due to potential differences in coexpression. In neurons, the activation of WNT7A by Frizzled-5 promotes synapse formation. It actively participates in the canonical Wnt/beta-catenin signaling pathway, leading to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and subsequent activation of Wnt target genes. Frizzled-5 may also engage a secondary signaling pathway involving PKC and calcium fluxes, the integration of which with the canonical pathway remains unclear, given PKC's apparent requirement for Wnt-mediated inactivation of GSK-3 kinase. This versatile receptor likely plays a role in transduction and intercellular transmission of polarity information during tissue morphogenesis and in differentiated tissues. Additionally, Frizzled-5 is implicated in yolk sac angiogenesis and placental vascularization, and its homodimerization is promoted by the binding of unsaturated fatty acid molecules via the FZ domain. Notably, Frizzled-5 interacts with WNT2B, WNT7A, and GOPC, contributing to its diverse range of cellular functions.