HIF-1 alpha Protein, Human (His)
Based on 4 publication(s) in Google Scholar
HIF-1 alpha is a key protein that responds to low oxygen levels. HIF-1 alpha Protein, Human (His) is the recombinant human-derived HIF-1 alpha protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HIF-1 alpha is a key protein that responds to low oxygen levels. HIF-1 alpha Protein, Human (His) is the recombinant human-derived HIF-1 alpha protein, expressed by E. coli , with N-His labeled tag.
Background
HIF-1 alpha functions as a master transcriptional regulator orchestrating the adaptive response to hypoxia, activating the transcription of over 40 genes critical for oxygen delivery, metabolic adaptation, and cellular response to low oxygen levels. Its target genes include erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and HILPDA. This protein plays essential roles in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic diseases. HIF-1 alpha forms a heterodimer with ARNT, binding to the hypoxia response element (HRE) in target gene promoters. Its activation requires coactivators such as CREBBP and EP300, and its activity is enhanced by interaction with NCOA1, NCOA2, and APEX1. Furthermore, HIF-1 alpha is involved in axonal distribution and mitochondrial transport in neurons during hypoxia and, upon infection by SARS-CoV-2, contributes to the induction of glycolysis and a pro-inflammatory state in monocytes. It induces the expression of ACE2 and cytokines, promoting SARS-CoV-2 replication and monocyte inflammatory response.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (4)
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Journal Impact Factor
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Most Recent
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Cancer Cell
2025 May 12;43(5):937-954.e9. PMID: 40054467 -
ACS Appl Mater Interfaces
Scab-Inspired Thermoresponsive Hydrogel Dressing for Accelerated Healing of Infected Wounds. [Abstract]2026 Jan 9. PMID: 41511899 -
Biochem Pharmacol
Baicalin promotes anti-tumor immunity in hepatocellular carcinoma through HIF-1α/Lactate/CXCL9 axis. [Abstract]2025 Nov:241:117157. PMID: 40659129 -
Int Immunopharmacol
Esketamine alleviates ferroptosis-mediated acute lung injury by modulating the HIF-1α/HO-1 pathway. [Abstract]2024 Sep 6;142(Pt A):113065. PMID: 39243557
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q16665-1 (R575-N826)
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Molecular Construction
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N-term
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6*His
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HIF-1 alpha (R575-N826)
Accession # Q16665-1 -
C-term
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Protein Length
Partial
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Synonyms
HIF1A; Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia Inducible Factor 1 Subunit Alpha; HIF-1-Alpha; BHLHe78; Hypoxia Inducible Factor 1 Alpha Subunit; PASD8; Hypoxia-Inducible Factor1alpha; MOP1; Member Of PAS Superfamily 1; Class E Basic Helix-Loop-Helix Protein 78; ARNT Interacting Protein; Hypoxia-Inducible Factor 1-Alpha; ARNT-Interacting Protein; PAS Domain-Containing Protein 8; HIF1-ALPHA; Member Of PAS Protein 1; HIF1-Alpha; HIF-1alpha; BHLHE78; HIF1; HIF-1A; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor)
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AA Sequence
RSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN
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Molecular Weight
Approximately 34 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 200 mM NaCl, 2 mM EDTA, pH 8.0, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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