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  5. IL-31 Protein, Human (HEK293, His)

IL-31 Protein, Human (HEK293, His)

Cat. No.: HY-P73209
COA Handling Instructions

IL-31, a multifunctional cytokine, activates STAT3, and potentially STAT1 and STAT5, through the IL-31 heterodimeric receptor (IL31RA and OSMR). Known for its involvement in skin immunity, IL-31 modulates immune responses. Beyond immunity, IL-31 enhances myeloid progenitor cell survival, indicating roles in diverse cellular processes. Moreover, IL-31 induces key gene expression, including RETNLA and serum amyloid A protein, in macrophages, suggesting broader impacts on inflammatory and immune-related pathways. IL-31 Protein, Human (HEK293, His) is the recombinant human-derived IL-31 protein, expressed by HEK293, with C-His labeled tag. The total length of IL-31 Protein, Human (HEK293, His) is 141 a.a., with molecular weight of ~25 kDa.

For research use only. We do not sell to patients.

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Description

IL-31, a multifunctional cytokine, activates STAT3, and potentially STAT1 and STAT5, through the IL-31 heterodimeric receptor (IL31RA and OSMR). Known for its involvement in skin immunity, IL-31 modulates immune responses. Beyond immunity, IL-31 enhances myeloid progenitor cell survival, indicating roles in diverse cellular processes. Moreover, IL-31 induces key gene expression, including RETNLA and serum amyloid A protein, in macrophages, suggesting broader impacts on inflammatory and immune-related pathways. IL-31 Protein, Human (HEK293, His) is the recombinant human-derived IL-31 protein, expressed by HEK293, with C-His labeled tag. The total length of IL-31 Protein, Human (HEK293, His) is 141 a.a., with molecular weight of ~25 kDa.

Background

IL-31, a cytokine with diverse functions, acts by activating STAT3, and potentially STAT1 and STAT5, utilizing the IL-31 heterodimeric receptor formed by IL31RA and OSMR. This activation mechanism has been implicated in skin immunity, highlighting its role in modulating immune responses. Additionally, IL-31 exhibits the capacity to enhance myeloid progenitor cell survival in vitro, emphasizing its involvement in cellular processes beyond immune regulation. Furthermore, IL-31 induces the expression of key genes, such as RETNLA and serum amyloid A protein, in macrophages, suggesting a broader impact on inflammatory and immune-related pathways.

Biological Activity

Data is not available.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q6EBC2/NP_001014358.1 (S24-T164)

Gene ID
Molecular Construction
N-term
IL-31 (S24-T164)
Accession # Q6EBC2
His
C-term
Synonyms
IL31; IL-31; Interleukin-31
AA Sequence

SHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT

Molecular Weight

Approximately 25 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-31 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-31 Protein, Human (HEK293, His)
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HY-P73209
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