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IL-31 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

IL-31 Protein activates STAT3, potentially STAT1 and STAT5, through the IL31 receptor. It enhances myeloid progenitor cell survival and induces RETNLA and serum amyloid A protein expression in macrophages. IL-31 Protein's diverse functions suggest its importance in biological processes and potential implications in immune regulation and cell survival. IL-31 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-31 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IL-31 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for IL-31 Protein, Mouse (HEK293, His)
  • IL-31 Protein, Mouse (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

IL-31 Protein activates STAT3, potentially STAT1 and STAT5, through the IL31 receptor. It enhances myeloid progenitor cell survival and induces RETNLA and serum amyloid A protein expression in macrophages. IL-31 Protein's diverse functions suggest its importance in biological processes and potential implications in immune regulation and cell survival. IL-31 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-31 protein, expressed by HEK293 , with C-His labeled tag.

Background

IL-31 Protein activates STAT3 and potentially STAT1 and STAT5 through the IL31 heterodimeric receptor, which consists of IL31RA and OSMR. It has been suggested to play a role in skin immunity and enhances the survival of myeloid progenitor cells in vitro. IL-31 Protein also induces the expression of RETNLA and serum amyloid A protein in macrophages. These diverse functions highlight the significance of IL-31 Protein in various biological processes and suggest its potential implications in immune regulation and cell survival.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-31 RA (HY-P77968) is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Mouse IL-31. The ED50 for this effect is 1-5 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 85%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-31 RA (HY-P77968) is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Mouse IL-31. The ED50 for this effect is 2.916 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q6EAL8/NP_083870.1 (T24-C163)

  • Gene ID
    76399  [NCBI]
  • Molecular Construction
    • N-term
    • IL-31 (T24-C163)
      Accession # Q6EAL8/NP_8387.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IL31; IL-31; Interleukin 31; Interleukin-31

  • AA Sequence

    TCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQLPCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLNLNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC

  • Predicted Molecular Mass

    17 kDa

  • Molecular Weight

    Approximately 19-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 85%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-31 RA (HY-P77968) is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Mouse IL-31. The ED50 for this effect is 2.916 μg/mL.

  • IL-31 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-31 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for IL-31 Protein, Mouse (HEK293, His)
  • IL-31 Protein, Mouse (HEK293, His) Product Image