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IL-34 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-34 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for IL-34 Protein, Mouse (HEK293, His)
  • IL-34 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag.

Background

Interleukin-34 (IL-34) is a cytokine with pivotal functions in innate immunity and inflammatory processes, as it promotes the proliferation, survival, and differentiation of monocytes and macrophages. Additionally, IL-34 plays a crucial role in the regulation of osteoclast proliferation and differentiation, influencing bone resorption. The cytokine's impact is mediated through its interaction with CSF1R, leading to the activation of downstream effectors and subsequent phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Structurally, IL-34 exists as a homodimer, and its intricate involvement in immune regulation and bone homeostasis highlights its significance in orchestrating cellular responses and maintaining tissue integrity.

Verified Bioactivity

1.Measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells and the ED50 is typically ≤200 ng/mL.
2.Measured by its ability to bind biotinylated recombinant mouse CSF1R in a functional ELISA.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 11.05 ng/mL. corresponding to a specific activity is 9.04×104 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q8R1R4-1 (N21-P235)

  • Gene ID
    76527  [NCBI]
  • Molecular Construction
    • N-term
    • IL-34 (N21-P235)
      Accession # Q8R1R4-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    IL34; Interleukin-34; Prev. C16orf77; Chromosome 16 Open Reading Frame 77; IL-34; Interleukin 34; MGC34647

  • AA Sequence

    NENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP

  • Predicted Molecular Mass

    26 kDa

  • Molecular Weight

    Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, 10% glycerol, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 11.05 ng/mL. corresponding to a specific activity is 9.04×104 units/mg.

  • IL-34 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-34 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for IL-34 Protein, Mouse (HEK293, His)
  • IL-34 Protein, Mouse (HEK293, His) Product Image