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  4. IL-34
  5. IL-34 Protein, Mouse (HEK293, His)

IL-34 Protein, Mouse (HEK293, His)

Cat. No.: HY-P73212
COA Handling Instructions

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-34 Protein, Mouse (HEK293, His) is 235 a.a., with molecular weight of ~38 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-34 Protein, Mouse (HEK293, His) is 235 a.a., with molecular weight of ~38 kDa.

Background

Interleukin-34 (IL-34) is a cytokine with pivotal functions in innate immunity and inflammatory processes, as it promotes the proliferation, survival, and differentiation of monocytes and macrophages. Additionally, IL-34 plays a crucial role in the regulation of osteoclast proliferation and differentiation, influencing bone resorption. The cytokine's impact is mediated through its interaction with CSF1R, leading to the activation of downstream effectors and subsequent phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Structurally, IL-34 exists as a homodimer, and its intricate involvement in immune regulation and bone homeostasis highlights its significance in orchestrating cellular responses and maintaining tissue integrity.

Biological Activity

1.Measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells and the ED50 is typically 50-200 ng/mL.
2.Measured by its ability to bind biotinylated recombinant mouse CSF1R in a functional ELISA.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q8R1R4 (M1-P235)

Gene ID

76527  [NCBI]

Molecular Construction
N-term
IL-34 (M1-P235)
Accession # Q8R1R4
His
C-term
Synonyms
Interleukin-34; IL-34; C16orf77
Molecular Weight

Approximately 38 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-34 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-34 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P73212
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