IRAK1 Protein, Human (Active, sf9, GST)

IRAK1 is a serine/threonine protein kinase that initiates innate immune responses against pathogens through Toll-like receptor (TLR) and IL-1R signaling. After TLR activation, IRAK1 is recruited by MYD88 and undergoes IRAK4 phosphorylation, autophosphorylation, and kinase activation. IRAK1 Protein, Human (sf9, GST) is the recombinant human-derived IRAK1 protein, expressed by sf9 insect cells , with N-GST labeled tag.

IRAK1, a serine/threonine-protein kinase, assumes a crucial role in initiating the innate immune response against foreign pathogens by participating in Toll-like receptor (TLR) and IL-1R signaling pathways. Upon TLR activation, IRAK1 is swiftly recruited by MYD88 to the receptor-signaling complex, leading to its phosphorylation by IRAK4, subsequent autophosphorylation, and activation of its kinase function. IRAK1 then phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2, and PELI3), promoting pellino-mediated polyubiquitination of IRAK1. The ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1, facilitating the assembly of the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. This cascade activates IKKs (CHUK/IKKA and IKBKB/IKKB), leading to NF-kappa-B nuclear translocation and activation. Additionally, IRAK1 phosphorylates TIRAP, promoting its ubiquitination and degradation. Furthermore, IRAK1 phosphorylates interferon regulatory factor 7 (IRF7), inducing its activation and translocation to the nucleus, thereby initiating the transcriptional activation of type I IFN genes, establishing an antiviral cellular state. When sumoylated, IRAK1 translocates to the nucleus and phosphorylates STAT3.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 25nM, the conversion rate is about 36%.

Human

Sf9 insect cells

N-GST

P51617 (R194-S712)

3654

Partial

RPFPFCWPLCEISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVKRLKENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDRLHCQTQACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLTPKLGDFGLARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVVLETLAGQRAVKTHGARTKYLKDLVEEEAEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPELGLGLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAGVPGHSEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS

Approximately 90 kDa, based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT, 0.1 M trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.