Leptin R/LEPR Protein, Mouse (HEK293, His)

Leptin R/LEPR protein, as a receptor for the hormone LEP/leptin, mediates central and peripheral effects by activating various signaling pathways (such as JAK2/STAT3 and MAPK cascade/FOS).In the hypothalamus, it regulates appetite, energy expenditure, bone mass, and hormone secretion.Leptin R/LEPR Protein, Mouse (HEK293, His) is the recombinant mouse-derived Leptin R/LEPR protein, expressed by HEK293 , with C-10*His labeled tag.

Leptin R/LEPR, the receptor for the hormone LEP/leptin, plays a pivotal role in mediating both central and peripheral effects of leptin through the activation of distinct signaling pathways, including JAK2/STAT3 and the MAPK cascade/FOS. In the hypothalamus, leptin functions as an appetite-regulating factor, inducing decreased food intake and increased energy consumption, thereby regulating bone mass and hypothalamo-pituitary-adrenal hormones. In the periphery, Leptin R/LEPR influences basal metabolism, reproductive function, pancreatic beta-cell function, insulin secretion, angiogenesis, and immune responses. It controls energy homeostasis and melanocortin production through STAT3 signaling, modulating various physiological processes such as fertility, growth, and glucose homeostasis. Additionally, Leptin R/LEPR is involved in the counter-regulatory response to hypoglycemia and has a specific impact on T lymphocyte responses, influencing cytokine production and T-cell proliferation. It may also play a role in transporting leptin across the blood-brain barrier and mediating its endocytosis without inducing STAT3 phosphorylation.

Immobilized Recombinant Mouse LEPR (C-10*His) at 5 μg/ml (100 μl/well) can bind Recombinant Mouse Leptin. Biotinylated by NHS-biotin prior to testing.The ED₅₀ of Recombinant Mouse Leptin is 1.34 ng/ml.

Mouse

HEK293

C-6*His

P48356-1 (L22-G839)

16847  [NCBI]

Extracellular Domain

LNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHCIYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGDVTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLTFLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAG

Approximately 120-150 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/ykg-1-cell.html