MEK1 Protein, Mouse (Inactive, sf9, His-GST)
Based on 1 Customer Validation
The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway.MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements.MEK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway.MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements.MEK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
MEK1 protein, a pivotal component of the MAP kinase signal transduction pathway, plays a crucial role in transducing extracellular signals into intracellular responses. Upon binding of ligands such as growth factors and cytokines to their cell-surface receptors, MEK1 is activated as part of the RAS-RAF1-MEK1/2-MAPK/ERK cascade. MEK1, along with MAP2K2/MEK2, catalyzes the dual phosphorylation of threonine and tyrosine residues in the activation loop of ERK1 and ERK2, thereby initiating downstream signaling events. This cascade regulates diverse biological functions, including cell growth, adhesion, survival, and differentiation. In addition to its role in the transcriptional and cytoskeletal regulation, the MAPK/ERK pathway impacts peroxisome proliferator-activated receptor gamma (PPARG), contributing to cellular processes such as differentiation and apoptosis. Furthermore, MEK1's involvement in endosomal dynamics and Golgi apparatus fragmentation during mitosis underscores its versatility in coordinating intricate cellular responses.
Verified Bioactivity
The product demonstrated no detectable kinase activity in an ATP/ADP-based kinase assay.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
P31938 (M1-I393)
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Molecular Construction
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N-term
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His-GST
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MEK1 (M1-I393)
Accession # P31938 -
C-term
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Protein Length
Full Length
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Synonyms
MAP2K1; MAPKK1; Prev. PRKMK1; MAPKK 1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MEK 1; MAPK/ERK Kinase 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); MEK1; CFC3; MKK1; MEL; ERK Activator Kinase 1; Mitogen-Activate
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AA Sequence
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCHVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI
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Predicted Molecular Mass
71.3 kDa
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Molecular Weight
Approximately 65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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