MFAP4 Protein, Human (HEK293, His-Flag)

MFAP4 is a protein that may be involved in calcium-dependent cell adhesion or cell-cell interactions and may play a role in elastic fiber assembly and maintenance. It exists as homodimers and is capable of forming higher oligomers. MFAP4 Protein, Human (HEK293, His-Flag) is the recombinant human-derived MFAP4 protein, expressed by HEK293 , with N-His, N-Flag labeled tag. The total length of MFAP4 Protein, Human (HEK293, His-Flag) is 234 a.a., with molecular weight of 38-45 kDa.

The MFAP4 protein is implicated in potential roles related to calcium-dependent cell adhesion and intercellular interactions. It may contribute to the assembly and maintenance of elastic fibers, as suggested by its interaction with proteins such as FBN1, FBN2, LOX, COL1A1, and ELN. MFAP4 forms homodimers and higher oligomers, and its interaction with collagen type I (COL1A1) occurs in a calcium-dependent manner. Additionally, MFAP4 interacts with elastin (ELN) in a calcium-dependent manner, promoting the self-assembly of ELN. These molecular interactions highlight the versatility of MFAP4 in participating in cellular adhesion, extracellular matrix dynamics, and elastic fiber-related processes.

Immobilized Human MFAP4, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC₅₀ ≤10.7 ng/mL determined by ELISA.

Human

HEK293

N-His;N-Flag

P55083 (V22-A255)

4239  [NCBI]

35-45 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.