Nicastrin Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag.
Background
Nicastrin, an indispensable subunit of the gamma-secretase complex, takes center stage in the intricate molecular machinery responsible for the endoproteolytic cleavage of integral membrane proteins. This includes pivotal substrates like Notch receptors and the amyloid-beta precursor protein (APP). The gamma-secretase complex, in which Nicastrin plays a crucial role, serves as a linchpin in the modulation of Notch and Wnt signaling cascades, exerting regulatory influence on downstream processes. In concert with other essential components, such as a presenilin homodimer, APH1, and PEN2, Nicastrin forms the functional gamma-secretase complex. Nicastrin's binding affinity extends to the proteolytically processed C-terminal fragments of APP, further underscoring its pivotal role in orchestrating the intricacies of this proteolytic machinery. Moreover, Nicastrin's interactions with presenilin components accentuate its integral role within the gamma-secretase complex.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
P57716 (G28-E668)
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Molecular Construction
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N-term
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Nicastrin (G28-E668)
Accession # P57716 -
His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
NCSTN; APH2; Nicastrin; Anterior Pharynx-Defective 2; KIAA0253; ATAG1874
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AA Sequence
GLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPTNTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELE
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Molecular Weight
Approximately 95-99 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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