PPM1A Protein, Human (His)
Based on 1 Customer Validation
PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.
PPM1A, an enzyme with broad specificity, functions as a negative regulator in various signaling pathways. It plays a pivotal role in the attenuation of TGF-beta signaling by dephosphorylating SMAD2 and SMAD3. This enzymatic action leads to the dissociation of SMAD2 and SMAD3 from SMAD4, their nuclear export, and ultimately, the termination of TGF-beta-mediated signaling. Additionally, PPM1A dephosphorylates PRKAA1 and PRKAA2, contributing to the modulation of cellular processes. Another significant aspect of its regulatory function involves the termination of TNF-alpha-mediated NF-kappa-B activation. In this context, PPM1A dephosphorylates and inactivates IKBKB/IKKB, contributing to the downregulation of NF-kappa-B signaling.
Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 1060.04 nmol/min/mg.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P35813-1 (G2-W382)
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Molecular Construction
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N-term
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PPM1A (G2-W382)
Accession # P35813-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
PPM1A; Protein Phosphatase 1A (Formerly 2C), Magnesium-Dependent, Alpha Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent 1A; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A; Protein Phosphatase 1A; Protein Phosphatase 2C, Alpha Isoform; PP2Calpha; Alternati
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AA Sequence
GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
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Predicted Molecular Mass
43.5 kDa
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Molecular Weight
Approximately 45 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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