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  3. Hydrolases (EC 3)
  4. PPM1A Protein, Human (His)

PPM1A Protein, Human (His)

Cat. No.: HY-P71227
SDS COA Handling Instructions

PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $90 In-stock
50 μg $270 In-stock
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Description

PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.

Background

PPM1A, an enzyme with broad specificity, functions as a negative regulator in various signaling pathways. It plays a pivotal role in the attenuation of TGF-beta signaling by dephosphorylating SMAD2 and SMAD3. This enzymatic action leads to the dissociation of SMAD2 and SMAD3 from SMAD4, their nuclear export, and ultimately, the termination of TGF-beta-mediated signaling. Additionally, PPM1A dephosphorylates PRKAA1 and PRKAA2, contributing to the modulation of cellular processes. Another significant aspect of its regulatory function involves the termination of TNF-alpha-mediated NF-kappa-B activation. In this context, PPM1A dephosphorylates and inactivates IKBKB/IKKB, contributing to the downregulation of NF-kappa-B signaling.

Biological Activity

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 1060.04 nmol/min/mg.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P35813-1 (G2-W382)

Gene ID
Molecular Construction
N-term
PPM1A (G2-W382)
Accession # P35813-1
6*His
C-term
Synonyms
Protein Phosphatase 1A; Protein Phosphatase 2C Isoform Alpha; PP2C-Alpha; Protein Phosphatase IA; PPM1A; PPPM1A
AA Sequence

GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW

Molecular Weight

Approximately 45.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PPM1A Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PPM1A Protein, Human (His)
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HY-P71227
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