2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. TGF-beta Superfamily
  5. Smad Family
  6. SMAD2
  7. SMAD2 Protein, Mouse (sf9, His-GST)

SMAD2 Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P73637
Handling Instructions

SMAD2 protein interacts with multiple partners, such as CREB-binding protein (CBP), EP300, SNON, and others. These interactions contribute to diverse cellular processes, including TGF-beta signaling and transcriptional regulation. SMAD2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SMAD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SMAD2 Protein, Mouse (sf9, His-GST) is 466 a.a., with molecular weight of ~90 kDa.

For research use only. We do not sell to patients.

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Description

SMAD2 protein interacts with multiple partners, such as CREB-binding protein (CBP), EP300, SNON, and others. These interactions contribute to diverse cellular processes, including TGF-beta signaling and transcriptional regulation. SMAD2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SMAD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SMAD2 Protein, Mouse (sf9, His-GST) is 466 a.a., with molecular weight of ~90 kDa.
Background

The protein CREB-binding protein (CBP) interacts with several other proteins, including EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, RANBP3, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-beta, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4L, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1, and IPO7. These interactions play roles in various cellular processes, including TGF-beta signaling and transcriptional regulation.
Species

Mouse
Source

Sf9 insect cells
Tag

N-His;N-GST
Accession

Q62432 (S2-S467)
Gene ID

17126  [NCBI]
Molecular Construction
N-term
His-GST
SMAD2 (S2-S467)
Accession # Q62432
C-term
Synonyms
Mothers against decapentaplegic homolog 2; MAD homolog 2; hMAD-2; SMAD2; MADH2; MADR2
Molecular Weight

Approximately 90 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SMAD2 Protein, Mouse (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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