SMAD2 Protein, Mouse (sf9, His-GST)

SMAD2 protein interacts with multiple partners, such as CREB-binding protein (CBP), EP300, SNON, and others.These interactions contribute to diverse cellular processes, including TGF-beta signaling and transcriptional regulation.SMAD2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SMAD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The protein CREB-binding protein (CBP) interacts with several other proteins, including EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, RANBP3, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-beta, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4L, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1, and IPO7. These interactions play roles in various cellular processes, including TGF-beta signaling and transcriptional regulation.

Mouse

Sf9 insect cells

N-His;N-GST

Q62432 (S2-S467)

17126  [NCBI]

Full Length of Mature Protein

SSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTANTVDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Approximately 90 kDa, based on SDS-PAGE under reducing conditions.

≥ 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP, 0.5 mM GSH, pH 8.0, 5% trehalose, 5% mannitol, 0.01% Tween-80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.