Syndecan-3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Syndecan-3 Protein, Human (HEK293, His) is a Dendritic cells (DCs)-specific heparan sulfate (HS) proteoglycan that captures HIV-1 through interaction with the HIV-1 envelope glycoprotein gp120. SDC3 is involved in the regulation of growth factor signalling, Notch and BMP signalling, adhesion and migration, proliferation and differentiation. Syndecan-3 is associated with the control of feeding behaviour and the generation of cerebellar fibrillar plaques in Alzheimer鈥檚 disease. Syndecan-3 Protein, Human (HEK293, His) is the recombinant human-derived Syndecan-3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Syndecan-3 Protein, Human (HEK293, His) is a Dendritic cells (DCs)-specific heparan sulfate (HS) proteoglycan that captures HIV-1 through interaction with the HIV-1 envelope glycoprotein gp120. SDC3 is involved in the regulation of growth factor signalling, Notch and BMP signalling, adhesion and migration, proliferation and differentiation. Syndecan-3 is associated with the control of feeding behaviour and the generation of cerebellar fibrillar plaques in Alzheimer鈥檚 disease[1][2][3]. Syndecan-3 Protein, Human (HEK293, His) is the recombinant human-derived Syndecan-3 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Syndecans (sdcs) are heparan sulphate proteoglycans (HSPG) composed of a core protein to which heparan sulphate (HS) glycosaminoglycan chains are covalently attached. These molecules form part of the glycocalyx, which comprises a network of membrane-bound proteoglycans and glycoproteins at the cell surface of endothelial cells. There are four mammalian syndecans, designated syndecan-1 (sdc-1), -2, -3, and -4. Sdc-3 is the predominant syndecan in the nervous system, where it was first identified, and has been associated with the control of feeding behaviour and the generation of cerebellar fibrillar plaques in Alzheimer’s disease. Sdc-3 is also an HSPG of the musculoskeletal system[1].
Syndecan-3 stabilizes the captured virus, enhances Dendritic cells (DCs) infection in cis, and promotes transmission to T cells. Removal of the HSs from the cell surface by heparinase III or by silencing syndecan-3 by siRNA partially inhibited HIV-1 transmission by immature DCs, whereas neutralizing both syndecan-3 and DC-SIGN completely abrogated HIV-1 capture and subsequent transmission. Syndecan-3 (SDC3) has the largest core protein of all four mammalian syndecans and can harbour both heparan sulphate and chondroitin sulphate chains[2][3].
Verified Bioactivity
Measured by the ability of the immobilized protein to inhibit the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 0.5-2.0 μg/ml in the presence of 5 μg/mL Fibronectin.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
ABM82826 (M1-K311)
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Molecular Construction
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N-term
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Syndecan-3 (M1-K311)
Accession # ABM82826 -
6*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
SDC3; Alternative Protein SDC3; Syndecan 3; Syndecan 3 (N-Syndecan); SYND3; Syndecan Neural Type; N-Syndecan; KIAA0468; Syndecan Proteoglycan 3; Syndecan; Syndecan-3; SDCN
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AA Sequence
MAIAYLGSSCPSQPPSSLALSLSPTPSDFEQESGIETAMRFSPDVALAVSTTPFEELPSERPTLEPATSPLVVTEVPEEPSQRATTVSTTMATTAATSTGDPTVATVPATVATATPSTPAAPPFTATTAVIRTTGVRRLLPLPLTTVATARATTPEAPSPPTTAAVLDTEAPTPRLVSTATSRPRALPRPATTQEPDIPERSTLPLGTTAPGPTEVAQTPTPETFLTTIRDEPEVPVSGGPSGDFELPEEETTQPDTANEVVAVGGAAAKASSPPGTLPKGARPGPGLLDNAIDSGSSAAQLPQKSILERK
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Molecular Weight
Approximately 58-100 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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