Copy Link Print Information

TrkB Protein, Canine (HEK293, His)

Customer Review

Based on 1 Customer Validation

TrkB Protein is a neurotrophic factor receptor and a member of the tropomyosin receptor kinase family. TrkB Protein has high affinity for brain-derived neurotrophic factor (BDNF) and plays a role in various physiological functions of neurons, including cell survival and differentiation. TrkB Protein has carcinogenic activity. TrkB Protein, Canine (HEK293, His) is the recombinant canine-derived TrkB protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Canine
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TrkB Protein, Canine (HEK293, His)
  • Experimental Validation Results for TrkB Protein, Canine (HEK293, His)
  • TrkB Protein, Canine (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

TrkB Protein is a neurotrophic factor receptor and a member of the tropomyosin receptor kinase family. TrkB Protein has high affinity for brain-derived neurotrophic factor (BDNF) and plays a role in various physiological functions of neurons, including cell survival and differentiation. TrkB Protein has carcinogenic activity. TrkB Protein, Canine (HEK293, His) is the recombinant canine-derived TrkB protein, expressed by HEK293 , with C-His labeled tag.

Background

TrkB protein has a high affinity for brain-derived neurotrophic factor (BDNF) and plays a role in various physiological functions of neurons, including cell survival and differentiation[1].
TrkB protein is involved in epithelial-mesenchymal transition associated with increased migration and invasion in many cancer cell lines[3].
Abnormal activation caused by overexpression or fusion of TrkB protein can promote the genesis, progression, and treatment resistance of various types of neurogenic tumors[5].
Dysregulation of TrkB protein is associated with the pathogenesis of many diseases, such as neurodegenerative diseases, angiogenesis, lung adenocarcinoma, and gastric cancer[2][3][4].

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Immobilized canine TrkB at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF. The ED50 for this effect is 69.78 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized canine TrkB at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF. The ED50 for this effect is 69.78 ng/mL.

Technical Parameters

  • Species Canine
  • Source HEK293
  • Tag C-His
  • Accession

    XP_541264.2 (C32-H430)

  • Gene ID
    484147  [NCBI]
  • Molecular Construction
    • N-term
    • TrkB (C32-H430)
      Accession # XP_541264.2
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NTRK2; Neurotrophic Tyrosine Kinase, Receptor, Type 2, Isoform CRA_a; Neurotrophic Receptor Tyrosine Kinase 2; Neurotrophic Tyrosine Kinase, Receptor, Type 2; TRKB; Neurotrophin Receptor Tyrosine Kinase Type 2; BDNF/NT-3 Growth Factors Receptor; BDNF-Trop

  • AA Sequence

    CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSADPENITEIYIANQKRLEIINEDDVEAYAGLKNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQETKSSPETQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNLYWDVGNLVSKHMNETSHMQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYKLVAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVADKSGREH

  • Molecular Weight

    Approximately 66-76 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized canine TrkB at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF. The ED50 for this effect is 69.78 ng/mL.

  • TrkB Protein, Canine (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TrkB Protein, Canine (HEK293, His)
  • Experimental Validation Results for TrkB Protein, Canine (HEK293, His)
  • TrkB Protein, Canine (HEK293, His) Product Image