TrkB Protein, Canine (HEK293, His)

TrkB Protein is a neurotrophic factor receptor and a member of the tropomyosin receptor kinase family. TrkB Protein has high affinity for brain-derived neurotrophic factor (BDNF) and plays a role in various physiological functions of neurons, including cell survival and differentiation. TrkB Protein has carcinogenic activity. TrkB Protein, Canine (HEK293, His) is the recombinant canine-derived TrkB protein, expressed by HEK293 , with C-His labeled tag.

TrkB protein has a high affinity for brain-derived neurotrophic factor (BDNF) and plays a role in various physiological functions of neurons, including cell survival and differentiation^[1].
TrkB protein is involved in epithelial-mesenchymal transition associated with increased migration and invasion in many cancer cell lines^[3].
Abnormal activation caused by overexpression or fusion of TrkB protein can promote the genesis, progression, and treatment resistance of various types of neurogenic tumors^[5].
Dysregulation of TrkB protein is associated with the pathogenesis of many diseases, such as neurodegenerative diseases, angiogenesis, lung adenocarcinoma, and gastric cancer^[2][3][4].

1.This product does not contain protein kinase domain.
2.Immobilized canine TrkB at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF. The ED₅₀ for this effect is 69.78 ng/mL.

• 
  Immobilized canine TrkB at 10 μg/mL (100 μL/well) can bind biotinylated mouse BDNF. The ED₅₀ for this effect is 69.78 ng/mL.

Canine

HEK293

C-His

XP_541264.2 (C32-H430)

484147  [NCBI]

Partial

CPTSCKCSASRIWCSDPSPGIVAFPRLEPNSADPENITEIYIANQKRLEIINEDDVEAYAGLKNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQETKSSPETQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNLYWDVGNLVSKHMNETSHMQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYKLVAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVADKSGREH

Approximately 60-78 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.