Vitronectin Protein, Mouse (HEK293, His)
Based on 1 publication(s) in Google Scholar
Vitronectin is a key cell adhesion factor in serum and tissues and plays a crucial role in cell interactions by binding to glycosaminoglycans and proteoglycans.It is recognized by specific integrins and serves as an important adhesion molecule, facilitating cell-matrix interactions.Vitronectin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vitronectin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Vitronectin is a key cell adhesion factor in serum and tissues and plays a crucial role in cell interactions by binding to glycosaminoglycans and proteoglycans.It is recognized by specific integrins and serves as an important adhesion molecule, facilitating cell-matrix interactions.Vitronectin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Vitronectin protein, expressed by HEK293 , with C-His labeled tag.
Vitronectin, a pivotal cell adhesion and spreading factor present in both serum and tissues, plays a crucial role in cellular interactions. It engages with glycosaminoglycans and proteoglycans, demonstrating its versatility in molecular interactions. Recognized by specific integrins, Vitronectin serves as a vital cell-to-substrate adhesion molecule, facilitating cellular adhesion processes. Additionally, it acts as an inhibitor, mitigating the membrane-damaging effects associated with the terminal cytolytic complement pathway. Notably, Vitronectin establishes connections with various molecules, including SERPINE1/PAI1, insulin, and C1QBP, showcasing its involvement in diverse cellular processes.
1.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells.
When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37°C.
2.Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (5 μg/mL and 100 μL/well), approximately is 79.89% adhered specifically after 30 minutes at 37°C.
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Nanotechnol
Spatiotemporal targeting of messenger RNA lipid nanoparticles to the endometrium for the treatment of reproductive disorders. [Abstract]2026 Jan 19. PMID: 41555025
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
P29788 (D20-K478)
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Molecular Construction
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N-term
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Vitronectin (M1-K478)
Accession # P29788 -
10*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
VTN; Vitronectin; VN; Serum Spreading Factor; Serum‑Spreading Factor; Complement S‑Protein; Somatomedin B; S‑Protein; V75; Vitronectin (Serum Spreading Factor, Somatomedin B, Complement S‑Protein); Epibolin; VNT
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AA Sequence
DQESCKGRCTQGFMASKKCQCDELCTYYQSCCADYMEQCKPQVTRGDVFTMPEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLNPRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPDTTDQGTPEFPEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWEYEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHGVPGKVDAAMAGRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSRSIWFSLFSSEESGLGTYNNYDYDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK
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Predicted Molecular Mass
54.2 kDa
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Molecular Weight
Approximately 75-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
3.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 6.5, 8% trehalose.
4.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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