Rhodamine B ethyl ester
Rhodamine B ethyl ester is a hydrophilic cationic derivative of Rhodamine B (HY-Y0016), belonging to non-selective cytotoxic agents and transporters. Rhodamine B ethyl ester exhibits broad-spectrum in vitro cytotoxicity and exerts cytotoxic effects on human tumor cells. Rhodamine B ethyl ester binds effectively to lipid membranes, undergoes voltage-dependent transport across planar bilayer phosphatidylcholine membranes, and its transport can be determined by current relaxation after voltage jump. Rhodamine B ethyl ester can be used in research related to cancers such as epithelioid melanoma and colorectal adenocarcinoma.
For research use only. We do not sell to patients.
- CAS No.: 2390-63-8
- Formula: C30H35ClN2O3
- Molecular Weight:507.06
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | EC50 |
0.38 μM
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Cytotoxicity against human epithelial melanoma A375 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against human epithelial melanoma A375 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
|
s00044-020-02591-8 |
| HT-29 | EC50 |
0.41 μM
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Cytotoxicity against human colorectal adenocarcinoma HT29 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against human colorectal adenocarcinoma HT29 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
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s00044-020-02591-8 |
| MCF7 | EC50 |
0.23 μM
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Cytotoxicity against human breast adenocarcinoma MCF-7 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against human breast adenocarcinoma MCF-7 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
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s00044-020-02591-8 |
| A2780 | EC50 |
0.21 μM
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Cytotoxicity against human ovarian carcinoma A2780 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against human ovarian carcinoma A2780 cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
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s00044-020-02591-8 |
| FaDu | EC50 |
0.30 μM
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Cytotoxicity against human squamous cell carcinoma FaDu cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against human squamous cell carcinoma FaDu cells assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
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s00044-020-02591-8 |
| NIH3T3 | EC50 |
0.96 μM
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Cytotoxicity against nonmalignant mouse NIH 3T3 fibroblasts assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
Cytotoxicity against nonmalignant mouse NIH 3T3 fibroblasts assessed by Sulforhodamine B (SRB) assay after 72 h incubation, averaged from three independent experiments performed in triplicate.
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s00044-020-02591-8 |
In Vitro
Rhodamine B ethyl ester (compound 1) exhibits cytotoxicity against human tumor cell lines A375, HT29, MCF-7, A2780 and FaDu, as well as non-malignant NIH 3T3 mouse fibroblasts, with EC50 values ranging from 0.21 μM (A2780) to 0.96 μM (NIH 3T3), and shows low selectivity[1].
Rhodamine B ethyl ester (C2RB) (0.2 μM) can translocate across DPhytanylPC planar bilayers, with a translocation rate constant of 1.1 s−1 and a characteristic relaxation time of 0.12 s under a 100 mV voltage jump[2].
Rhodamine B ethyl ester shows slower translocation rates in ester-type planar bilayer membranes (DPhPC, DOPC, DEPC) than in ether-linked DPhytanylPC bilayers, and its characteristic relaxation time rises as the fatty acid chains of membrane lipids get longer under a 100 mV voltage jump[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2390-63-8
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Molecular Weight 507.06
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Formula C30H35ClN2O3
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SMILES
O=C(C1=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C=CC=C1)OCC.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Rhodamine B ethyl ester Related Classifications
HY-187116 Related Classifications
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