Roseoside 

Roseoside is an inhibitor of DNA gyrase and HAV 3C protease, and also inhibits HCV NS5A/B replicase in human systems with an IC₅₀ of 20 μM. Roseoside binds to the active site of enzymes and stabilizes the interaction by forming hydrogen bonds with key amino acid residues. Roseoside inhibits the growth of Gram-positive bacteria, Gram-negative bacteria, and Candida albicans, and interferes with HCV RNA replication in vitro by inhibiting HCV NS5A/B replicase (IC₅₀=20 μM). Roseoside shows no cytotoxicity and serves as a research tool for studies related to bacterial infections, candidiasis, HAV and HCV^[1][2].

Roseoside potently inhibits the growth of Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and Candida albicans with MIC values of 4.53 µM, 9.05 µM, 3.23 µM, 5.56 µM, and 2.59 µM, respectively^[1].
Roseoside (31.25-1000 µg/mL) exhibits low cytotoxicity toward Vero cells, with an IC₅₀ of 591.50 µg/mL and a maximum non-toxic concentration of 250 µg/mL^[1].
Roseoside exhibits favorable in silico ADME properties, including moderate gastrointestinal absorption, no blood-brain barrier permeability, and a bioavailability score of 0.55, supporting its potential as an orally bioactive agent^[1].
Roseoside (40 μM; 48 h) significantly inhibits HCV infection in naïve Huh7.5 cells, reducing relative HCV RNA levels to 48% of the control^[2].
Roseoside (5-40 μM; 48 h) inhibits HCV infection in naïve Huh7.5 cells in vitro in a concentration-dependent manner (EC₅₀ = 38.92 μM over 48 h) with no marked cytotoxicity at concentrations ≤40 μM^[2].
Roseoside (40 μM; maintained from 4 h post-inoculation) inhibits HCV replication in naïve Huh7.5 cells in a time-dependent manner, reducing intracellular HCV RNA to 62% and 49% of the control at 36 and 48 h post-inoculation, respectively^[2].
Roseoside (20-40 μM; 15 min pre-incubation at room temperature, followed by 1 h reaction) inhibits HCV NS5A/B replicase activity in vitro, reducing activity to 50% and 45% of the control at concentrations of 20 μM and 40 μM, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

386.44

C₁₉H₃₀O₈

54835-70-0

Solid

White to off-white

C[C@@H](O[C@@H]([C@@H]([C@H]1O)O)O[C@@H]([C@H]1O)CO)/C=C/[C@](C(C)(C2)C)(C(C)=CC2=O)O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Mohamed M F S, et al. Bioactive potential of Latania verschaffeltii Lem. leaves (Arecaceae): phytochemical, antimicrobial, antiviral, and computational evaluation[J]. The Science of Nature, 2026, 113(2): 40.

  [2]. Lee J K, et al. Roseoside Is a Bioactive Compound in Kirengeshoma koreana Nakai Extract with Potent In Vitro Antiviral Activity Against Hepatitis C Virus[J]. Molecules, 2024, 29(21): 5130.